Detailed information of TA system TA189089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3110715..3111319
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: JN148_RS14785
• Protein ID: WP_003414492.1
• Coordinates: 3110715..3111107 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN148_RS14790
• Protein ID: WP_003910640.1
• Coordinates: 3111104..3111319 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS14755	3105865..3106653	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
JN148_RS14760	3106987..3107532	-	546	WP_031667139.1	DUF1802 family protein	-
JN148_RS14765	3107804..3108688	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JN148_RS14770	3108691..3109578	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
JN148_RS14775	3109883..3110428	-	546	WP_013988952.1	DUF1802 family protein	-
JN148_RS14780	3110425..3110694	-	270	WP_003414489.1	DUF2277 family protein	-
JN148_RS14785	3110715..3111107	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN148_RS14790	3111104..3111319	-	216	WP_003910640.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
JN148_RS14795	3111366..3112115	+	750	WP_003414497.1	enoyl-CoA hydratase	-
JN148_RS14800	3112194..3113276	-	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
JN148_RS14805	3113269..3114579	-	1311	WP_013988953.1	ABC transporter substrate-binding protein	-
JN148_RS14810	3114582..3115409	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
JN148_RS14815	3115406..3116317	-	912	WP_013988954.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T189089 WP_003414492.1 NZ_CP069065:c3111107-3110715 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T189089 NZ_CP090636:c1650877-1650617 [Burkholderia contaminans]
ATGCGCACGATTCATTTTTCGGATGCCCGTGGCAATCTGAAGACCGTTATCGACCAGGTCGTCGATGATGCGGACGTGAC
GCTCATCACGCGCCGCGATGCGCCGAATGCCGTCATCATGTCGCAGGATTACTACGACAGCCTGATGGAGACGGTTCACT
TGCTGCGGTCTCCGGCCAACGTCGCCCATCTCGAACGTTCGATCGCGCAACTGCGCAAGGGCAAGGCGAAAGAACACAAG
CTCGCGGCGGATGACGAATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7495.55 Da
• Isoelectric Point: 4.9732

>AT189089 WP_003910640.1 NZ_CP069065:c3111319-3111104 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVAWNVS

Nucleotide

• Length: 216 bp

>AT189089 NZ_CP090636:c1650620-1650354 [Burkholderia contaminans]
ATGAGCGTACGCCGCGTGCTCTTTACTTCCGACGCGTGGGACGACTATGTCTACTGGCAGGGGCAGGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAGCCCAGCGAACGCCGTTCGAAGGCATCGGCAAACCCGAACCGTTAAAGGCCA
ATCTCTCCGGCTTCTGGTCGCGCCGTATCGACGACACGAACCGTCTGGTCTACGAGGCCGACGACTCGCAGATCAGCATC
GTGTCCTGCCGCTACCACTACGAATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8