Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3082918..3083488 Replicon chromosome
Accession NZ_CP069065
Organism Mycobacterium tuberculosis strain N0091

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN148_RS14640 Protein ID WP_003414166.1
Coordinates 3082918..3083274 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN148_RS14645 Protein ID WP_003901465.1
Coordinates 3083258..3083488 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN148_RS14620 3078370..3080058 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN148_RS14625 3080062..3080388 - 327 WP_003414157.1 hypothetical protein -
JN148_RS14630 3080561..3081148 + 588 WP_003914429.1 DUF3558 family protein -
JN148_RS14635 3081167..3082816 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN148_RS14640 3082918..3083274 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN148_RS14645 3083258..3083488 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN148_RS14650 3083531..3084574 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN148_RS14655 3084573..3085040 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN148_RS14660 3085216..3085470 - 255 WP_003917684.1 hypothetical protein -
JN148_RS14665 3085618..3086022 + 405 WP_003414181.1 hypothetical protein -
JN148_RS14670 3086019..3086210 + 192 WP_003414184.1 hypothetical protein -
JN148_RS14675 3086427..3086687 + 261 Protein_2897 transposase -
JN148_RS14680 3087797..3088054 + 258 WP_003899489.1 hypothetical protein -
JN148_RS14685 3088159..3088470 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189087 WP_003414166.1 NZ_CP069065:c3083274-3082918 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189087 NZ_CP090636:917388-917669 [Burkholderia contaminans]
ATGACCTTTGAGCTTGCCTTTCTCGAACCTGCTCTGAAAGAGTGGAAGAAGCTCGATCGCACCGTTCGCGATCAGTTCAA
ATCCCGGCTCGCCGAGCGGCTCGAGAATCCTCGAATACCGTCCGCGAAGCTGCAGGGTCATCCGGACCGCTACAAGATCA
AGCTGCGCAGCGTCGGCTATCGGCTCGTGTACGAAGTGCGCGATTCGGAAGTGCTCGTACTGGTCGTGGCGGTCGGTCGA
CGCGAGCGCGATGCTGTCTATCTTGCCGCGATGAAGCGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189087 WP_003901465.1 NZ_CP069065:c3083488-3083258 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189087 NZ_CP090636:917150-917398 [Burkholderia contaminans]
ATGCCTCATACCATTCTGGCCAACGTTACGGCGAGCGTTTCGGAACTGAAGCGGAATCCGATGGGCACGGTCGCGGCAGG
AGAGGGTTTCCCGGTCGCGATCCTGAATCACAACGAACCCGCGTTCTATTGCATCCCGGCGAAGACCTGGGAGGCGATGG
TCGAACGCCTTGAAGATATCGAAGACAACGCGTTGGCCGACAAGCGCGCCAAGGAGAAGGTCGTCAAGGTCAAGCTGAAT
GACCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References