Detailed information of TA system TA189085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3042922..3043601
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN148_RS14410
• Protein ID: WP_003414059.1
• Coordinates: 3042922..3043338 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN148_RS14415
• Protein ID: WP_003414061.1
• Coordinates: 3043335..3043601 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS14385	3038974..3039876	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN148_RS14390	3039945..3040697	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN148_RS14400	3040941..3041216	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN148_RS14405	3041213..3042835	-	1623	WP_013988948.1	class I SAM-dependent DNA methyltransferase	-
JN148_RS14410	3042922..3043338	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN148_RS14415	3043335..3043601	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN148_RS14420	3043627..3044022	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN148_RS14425	3044019..3044288	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN148_RS14430	3044298..3045392	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN148_RS14435	3045389..3045808	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN148_RS14440	3045807..3045881	+	75	Protein_2850	hypothetical protein	-
JN148_RS14445	3045882..3046361	-	480	WP_003414073.1	dihydrofolate reductase	-
JN148_RS14450	3046432..3047232	-	801	WP_003911953.1	thymidylate synthase	-
JN148_RS14455	3047388..3048125	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189085 WP_003414059.1 NZ_CP069065:c3043338-3042922 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189085 NZ_CP090635:3363594-3363995 [Burkholderia contaminans]
ATGCCGCGCTACATGCTCGACACCAACATGTGCATCTACCTGATGAAGAATCAGCCGGAGCAGGTTGCGAGACGGTTTGC
GCAGTGCTACACGGGGGACGTCGTGATGTCGGCGATCACGTACGCCGAGCTCGAGTACGGTGTCGCGGCGTGCGCGAGCC
CTGCGCGGGAGCGCGGCAATCTTGCCGCGCTGATCGACGACATTCCCGTTGCGCCGTTCGACGTCGCGGCCGCGCAAGCG
TATGGTCCGGTCCGCGAGGCAACCCGGGAGCGGAAGAAGGATCACCTCGACAAGCTGATCGCCGCGCATGCGGTGTCGCT
CGATGTCGTACTCGTGACCAACAACGAGCGGGATTTCGTGAGCTATCCGGGATTGCGGCTGGAGAACTGGCTGGAGGACT
GA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189085 WP_003414061.1 NZ_CP069065:c3043601-3043335 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189085 NZ_CP090635:3363358-3363594 [Burkholderia contaminans]
ATGCATACGACGAGGGTATTTCGGAACGGCAACTCGCAGGCCGTGCGAATTCCTGCCGACCTTGCCTATGAACGCAGCGA
TATCGAACTGGAAATCGAGCGCATTGGTGACGAAATCCGCATTCGGCCGGCGCGGCGGCCGTTGACCCGCGTCCTTGAGA
AATTCGCTAAATTCGGGCCGGATTTCATGGCGGATGGGCGCGGTGACCACGAGCAGGCCGATCGCGAGGGTTTGTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64