Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2853464..2854101 Replicon chromosome
Accession NZ_CP069065
Organism Mycobacterium tuberculosis strain N0091

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN148_RS13390 Protein ID WP_003413180.1
Coordinates 2853464..2853859 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN148_RS13395 Protein ID WP_003413183.1
Coordinates 2853856..2854101 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN148_RS13350 2849549..2850208 + 660 WP_003900846.1 LppA family lipoprotein -
JN148_RS13355 2850205..2850864 + 660 WP_003900847.1 LppA family lipoprotein -
JN148_RS13360 2850861..2851523 + 663 WP_003900848.1 LppA family lipoprotein -
JN148_RS13365 2851520..2851798 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN148_RS13370 2851891..2852304 + 414 WP_003413164.1 PIN domain nuclease -
JN148_RS13375 2852343..2852600 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN148_RS13380 2852597..2852974 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN148_RS13385 2852990..2853364 - 375 WP_003413177.1 hypothetical protein -
JN148_RS13390 2853464..2853859 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN148_RS13395 2853856..2854101 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN148_RS13400 2854512..2854931 - 420 WP_003413190.1 A24 family peptidase -
JN148_RS13405 2854943..2855751 - 809 Protein_2648 shikimate dehydrogenase -
JN148_RS13410 2855748..2857001 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN148_RS13415 2856994..2857506 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189083 WP_003413180.1 NZ_CP069065:c2853859-2853464 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189083 NZ_CP090635:1404898-1405137 [Burkholderia contaminans]
ATTTGGCGCGATACCGTCGGGCGTGGGTCGCTCAAGCAAGCCGACCTTTACCACCGCGATCTGATCGGAACCATGGAAGC
GCTGGCGCGCGGTGAAAAGGTCGGCCGGACTTGCATTGTTCGCGATGGATATTTCCGGTACGCCGTGGGCTCCCACGTCG
TGTTCTATCGTGAAACGGAATACACGATCGATTTGATTCGGGTCCTGCATCAACGCATGGACATCGAACAACATCTGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189083 WP_003413183.1 NZ_CP069065:c2854101-2853856 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189083 NZ_CP090635:1404607-1404852 [Burkholderia contaminans]
ATGTCGCAAAACACATCCGTTTCCCGGCGCGACCACTTTGCCGAACTCGTCGATACCCAAGTGCAATCGGAACAATACGG
TTCCGCAAGCGACGTCGAACGAACCGGTTTGCGGCTTCTCGAATCACGCGACATGCAGGTGCGCGCGTTGCAGGAAGCGT
TGAAGGCCGGGGAGGTATCGGGAGCACCCAGGCCATTCGACCGCGAGGCCTTCCTGGCCCGCATGCGGGCCGCGCATGAC
GGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References