Detailed information of TA system TA189082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2852343..2852974
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: JN148_RS13380
• Protein ID: WP_003413174.1
• Coordinates: 2852597..2852974 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN148_RS13375
• Protein ID: WP_003413167.1
• Coordinates: 2852343..2852600 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS13340	2847601..2847915	+	315	WP_009937839.1	hypothetical protein	-
JN148_RS13345	2848211..2849422	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN148_RS13350	2849549..2850208	+	660	WP_003900846.1	LppA family lipoprotein	-
JN148_RS13355	2850205..2850864	+	660	WP_003900847.1	LppA family lipoprotein	-
JN148_RS13360	2850861..2851523	+	663	WP_003900848.1	LppA family lipoprotein	-
JN148_RS13365	2851520..2851798	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN148_RS13370	2851891..2852304	+	414	WP_003413164.1	PIN domain nuclease	-
JN148_RS13375	2852343..2852600	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN148_RS13380	2852597..2852974	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN148_RS13385	2852990..2853364	-	375	WP_003413177.1	hypothetical protein	-
JN148_RS13390	2853464..2853859	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN148_RS13395	2853856..2854101	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN148_RS13400	2854512..2854931	-	420	WP_003413190.1	A24 family peptidase	-
JN148_RS13405	2854943..2855751	-	809	Protein_2648	shikimate dehydrogenase	-
JN148_RS13410	2855748..2857001	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN148_RS13415	2856994..2857506	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T189082 WP_003413174.1 NZ_CP069065:2852597-2852974 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T189082 NZ_CP090635:1401076-1401237 [Burkholderia contaminans]
GTGACCACGCTCTACATGCTCGATACGAGCATCTGTTCGTTCATCATGCGCGAGCGGCCGCCCGTGGTGCTGTCCCGGTT
GCAATCGTGCGTCAATGCGCAGCACCGGATCGTCGTCTCGGCAATTACCTATGCCGAAATGCGATTCGGCGCGGCCGGCT
AG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189082 WP_003413167.1 NZ_CP069065:2852343-2852600 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189082 NZ_CP090635:1400813-1401079 [Burkholderia contaminans]
ATGCGCACCGTTTCCATTTTCAAGAATGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
CGAACTCGAGATTCGCCGCGAGGGCGACACGCTGCTGCTGCGGCCGGTGCGGCCCACATGGCTCTCGTTCGCGAGCGAAC
CGCCGGCCGACGCCGACTTCCTCGCCGAACGCCCGGCCGTCATCGAGCCCGGCCGCTTCGACCTGTCCGACGGTGGCGGC
GCCGCACCGACGGAGTCCGGCCAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5