Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2309911..2310547 Replicon chromosome
Accession NZ_CP069065
Organism Mycobacterium tuberculosis strain N0091

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag JN148_RS10795 Protein ID WP_003410654.1
Coordinates 2310137..2310547 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag JN148_RS10790 Protein ID WP_003410651.1
Coordinates 2309911..2310144 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN148_RS10775 2305368..2305760 + 393 WP_229303073.1 metal ABC transporter permease -
JN148_RS10780 2305761..2306165 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
JN148_RS10785 2306249..2309833 - 3585 WP_013988897.1 cobaltochelatase subunit CobN -
JN148_RS10790 2309911..2310144 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
JN148_RS10795 2310137..2310547 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
JN148_RS10800 2310531..2311622 + 1092 WP_003900454.1 precorrin-3B synthase -
JN148_RS10805 2311632..2312258 + 627 WP_003410658.1 precorrin-8X methylmutase -
JN148_RS10810 2312255..2313781 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
JN148_RS10815 2313727..2314950 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T189076 WP_003410654.1 NZ_CP069065:2310137-2310547 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T189076 NZ_CP090633:1295930-1296349 [Burkholderia contaminans]
ATGATCCTTGTTGATACGAACGTGATGTCCGAGCCGCTGCGGCGTGAGCCGAGCGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAACGTCGAAACGTTGTTTCTTGCTGCGATCAGTCTTGGCGAAATGCGCTTCGGCGTGGCGGCGCTCCCGGAAGGGC
GCAGGCGCGACTGGCTTCACCACAGCATCGAGCAACGCGTCGTGCCGCTGTTTCGCGGCAGGATTCTGCCGTTCGACGAT
GCGGCGAGCAAAGCGTATGCGAGCATTCGAGCGCGTGCGCGTGCCTCGGGCAGTACGATCGCGCCTGCAGATGTCTTTAT
TGCCGCGACGGCCGAGGCGAACGGATTGATCATTGCGACGCGCGACGTTGCGCCATTCGAGGCTGTCGGCCTGCGGGTGA
TTGATCCATGGGCGTCGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT189076 WP_003410651.1 NZ_CP069065:2309911-2310144 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT189076 NZ_CP090633:1295694-1295933 [Burkholderia contaminans]
ATGCCCGTGATTACTGTTCGAAATTTGCCCGATGAAGTGCACCGCGCGCTCCGAATTCGTGCGGCGCAGCATGGGCGTAG
CACTGAAGCCGAGGTGCGTGACATCCTGGAGCAGGCCGTTCTGCCGGGCGGCCGGCTGAAGCTGGGAACGCTGCTGGCTG
AAATCGGACGGGAGGCGGGCGGCGTCGAATTCGACGTTCGGCGCGACAAGACGCTAACCGACCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References