Detailed information of TA system TA189074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2242127..2242753
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN148_RS10505
• Protein ID: WP_003410075.1
• Coordinates: 2242355..2242753 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN148_RS10500
• Protein ID: WP_003911750.1
• Coordinates: 2242127..2242354 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS10490	2240166..2240510	-	345	WP_003410065.1	ferredoxin family protein	-
JN148_RS10495	2240699..2241868	-	1170	WP_003899126.1	ATP-binding protein	-
JN148_RS10500	2242127..2242354	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN148_RS10505	2242355..2242753	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JN148_RS10510	2242936..2243367	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN148_RS10515	2243468..2243902	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN148_RS10520	2244339..2244518	-	180	Protein_2080	hypothetical protein	-
JN148_RS10525	2244606..2245226	+	621	WP_003410086.1	IS110 family transposase	-
JN148_RS10530	2245180..2245770	+	591	WP_003899131.1	IS110 family transposase	-
JN148_RS10535	2245898..2247154	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T189074 WP_003410075.1 NZ_CP069065:2242355-2242753 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T189074 NZ_CP090632:c1450503-1450141 [Burkholderia contaminans]
ATGGATCCATACATCAAACCGCTGTACTGGCTCGGCTCAGCGCGCAAGGATTTGAAAGCGATGCCGCAAGCCGTGCAGGA
CACGTTCGGCTATGCGCTGTATCTGGCGCAGACGGGACGCAAGCACGATCAGGCCAAGCCGTTGAAAGGATTCGGCTCGG
CCGGCGTGCTCGAGATCGTTGAATCGGAAGACAACGGCACGTATCGCGCGGTCTATACGGTGAAGCTCGGCAGCAGCGTG
TACGTGCTGCACTGTTTCCAGAAGAAGTCGTCGATCGGTATCGCAACACCGAGGCCGGACAGCGATCTCGTCCGCGAACG
TCTGAAAGCGGCCGAAGCACACGCACAAGGAGTAAGGCGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT189074 WP_003911750.1 NZ_CP069065:2242127-2242354 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT189074 NZ_CP090632:c1450144-1449821 [Burkholderia contaminans]
ATGATCGAAATCGAACAGGGCAGCGGCAACGTCTACGCCGATCTCGGCATGCACGATGCCGCCGAAATGCGGGTGAAAGC
CCAACTCGCCGCGAAGATCGCGGAAATCATCAAGGCGCGCCAATGGACACAGCAGCACGCGGCCCGGTTGCTCGGCATCA
CGCAGCCGAAGCTGTCGAACATGCTGCGCGGGCAGTTTCGCGGCGTCAGCGAAACGAAGATGCTCGAATGTCTGGCGAAG
CTCGGACGCGACGTGCACATCGTCGTCGGGCCCGATCGTCAATCGGAAGTCGAAGGGCACATCGAGGTCGTCTTCGCAGC
GTGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8