Detailed information of TA system TA189072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2216828..2217726
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: -
• Locus tag: JN148_RS10380
• Protein ID: WP_244692953.1
• Coordinates: 2216828..2217388 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: JN148_RS10385
• Protein ID: WP_003410003.1
• Coordinates: 2217385..2217726 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS10350	2211997..2212650	-	654	WP_003409976.1	cutinase Cfp21	-
JN148_RS10355	2212765..2212995	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JN148_RS10360	2213080..2213991	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN148_RS10365	2214100..2214699	+	600	WP_003409989.1	L-lysine exporter	-
JN148_RS10370	2215115..2215543	+	429	WP_003409992.1	cellulose-binding protein	-
JN148_RS10375	2215769..2216308	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN148_RS10380	2216828..2217388	-	561	WP_244692953.1	RES domain-containing protein	Toxin
JN148_RS10385	2217385..2217726	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
JN148_RS10390	2217812..2218069	+	258	WP_003410006.1	hypothetical protein	-
JN148_RS10395	2217970..2218305	-	336	WP_003410009.1	dehydrogenase	-
JN148_RS10400	2218394..2218738	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JN148_RS10405	2218732..2218980	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JN148_RS10410	2219080..2221395	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
JN148_RS10415	2221392..2221664	-	273	WP_003410017.1	DUF1490 family protein	-
JN148_RS10420	2221717..2222073	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20306.74 Da
• Isoelectric Point: 4.4390

>T189072 WP_244692953.1 NZ_CP069065:c2217388-2216828 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPDQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T189072 NZ_CP090629:c5363884-5363501 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT189072 WP_003410003.1 NZ_CP069065:c2217726-2217385 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT189072 NZ_CP090629:c5364126-5363884 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAE4