Detailed information of TA system TA189068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2191451..2192154
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN148_RS10185
• Protein ID: WP_003409778.1
• Coordinates: 2191451..2191780 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN148_RS10190
• Protein ID: WP_003409780.1
• Coordinates: 2191777..2192154 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS10165	2187834..2188904	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN148_RS10170	2188901..2189416	+	516	WP_003409718.1	flavin reductase family protein	-
JN148_RS10175	2189413..2190474	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN148_RS10180	2190471..2191241	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN148_RS10185	2191451..2191780	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN148_RS10190	2191777..2192154	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN148_RS10195	2192151..2192741	-	591	WP_003910477.1	SEC-C metal-binding domain-containing protein	-
JN148_RS10200	2192796..2194160	+	1365	WP_013988890.1	HNH endonuclease signature motif containing protein	-
JN148_RS10205	2194315..2194767	-	453	WP_003899095.1	lipoprotein	-
JN148_RS10210	2194831..2195232	+	402	WP_003409869.1	hypothetical protein	-
JN148_RS10215	2195225..2195407	-	183	WP_003409870.1	hypothetical protein	-
JN148_RS10220	2195521..2195871	-	351	WP_003910480.1	hypothetical protein	-
JN148_RS10225	2195882..2196784	-	903	WP_003409874.1	hypothetical protein	-
JN148_RS10230	2196805..2196996	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189068 WP_003409778.1 NZ_CP069065:c2191780-2191451 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189068 NZ_CP090629:4163465-4163683 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189068 WP_003409780.1 NZ_CP069065:c2192154-2191777 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189068 NZ_CP090629:4163064-4163438 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8