Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1687592..1688208 Replicon chromosome
Accession NZ_CP069065
Organism Mycobacterium tuberculosis strain N0091

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JN148_RS07945 Protein ID WP_003407593.1
Coordinates 1687891..1688208 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A109SWY9
Locus tag JN148_RS07940 Protein ID WP_003910377.1
Coordinates 1687592..1687894 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN148_RS07930 1683478..1685325 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JN148_RS07935 1685326..1687578 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JN148_RS07940 1687592..1687894 + 303 WP_003910377.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JN148_RS07945 1687891..1688208 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JN148_RS07950 1688205..1689209 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JN148_RS07955 1689262..1690551 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
JN148_RS07960 1690624..1691241 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
JN148_RS07965 1691455..1691646 - 192 WP_003407609.1 dodecin family protein -
JN148_RS07970 1691728..1692126 + 399 WP_003900351.1 hypothetical protein -
JN148_RS07975 1692171..1693199 + 1029 WP_013988849.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T189064 WP_003407593.1 NZ_CP069065:1687891-1688208 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T189064 NZ_CP090629:740212-740697 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11203.45 Da        Isoelectric Point: 8.5236

>AT189064 WP_003910377.1 NZ_CP069065:1687592-1687894 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARAWRNARQQRSAQ

Download         Length: 303 bp

>AT189064 NZ_CP090629:739922-740215 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A109SWY9

References