Detailed information of TA system TA189063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1575050..1575705
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7U4FAR1
• Locus tag: JN148_RS07445
• Protein ID: WP_003407268.1
• Coordinates: 1575050..1575451 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN148_RS07450
• Protein ID: WP_244693195.1
• Coordinates: 1575448..1575705 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS07430	1570522..1571907	-	1386	WP_003407260.1	cytochrome P450	-
JN148_RS07435	1571985..1573019	+	1035	WP_003407264.1	AraC family transcriptional regulator	-
JN148_RS07440	1573065..1574795	-	1731	WP_013988836.1	PE family protein	-
JN148_RS07445	1575050..1575451	-	402	WP_003407268.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN148_RS07450	1575448..1575705	-	258	WP_244693195.1	CopG family transcriptional regulator	Antitoxin
JN148_RS07455	1575788..1576747	-	960	WP_003407276.1	alpha/beta hydrolase	-
JN148_RS07460	1576772..1577734	-	963	WP_003407279.1	alpha/beta hydrolase	-
JN148_RS07465	1577733..1578470	+	738	WP_031647000.1	lysoplasmalogenase	-
JN148_RS07470	1578551..1580518	+	1968	WP_003407285.1	primosomal protein N'	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14894.39 Da
• Isoelectric Point: 11.6897

>T189063 WP_003407268.1 NZ_CP069065:c1575451-1575050 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Nucleotide

• Length: 402 bp

>T189063 NZ_CP090629:352721-353068 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9413.56 Da
• Isoelectric Point: 6.4631

>AT189063 WP_244693195.1 NZ_CP069065:c1575705-1575448 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTAGLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Nucleotide

• Length: 258 bp

>AT189063 NZ_CP090629:353068-353367 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FAR1