Detailed information of TA system TA189060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1380729..1381417
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: JN148_RS06605
• Protein ID: WP_003406304.1
• Coordinates: 1380986..1381417 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: JN148_RS06600
• Protein ID: WP_003406302.1
• Coordinates: 1380729..1380989 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS06580	1376306..1377130	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
JN148_RS06585	1377135..1378316	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
JN148_RS06590	1378393..1379493	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
JN148_RS06595	1379664..1380653	+	990	WP_003910306.1	malate dehydrogenase	-
JN148_RS06600	1380729..1380989	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
JN148_RS06605	1380986..1381417	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN148_RS06610	1381440..1383127	-	1688	Protein_1306	PE family protein	-
JN148_RS06615	1383307..1384167	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
JN148_RS06620	1384248..1385078	-	831	WP_003910309.1	SDR family oxidoreductase	-
JN148_RS06625	1385135..1385428	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
JN148_RS06630	1385425..1385694	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189060 WP_003406304.1 NZ_CP069065:1380986-1381417 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189060 NZ_CP090626:c5364842-5364459 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189060 WP_003406302.1 NZ_CP069065:1380729-1380989 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189060 NZ_CP090626:c5365084-5364842 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6