Detailed information of TA system TA189056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1021183..1022096
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: JN148_RS04880
• Protein ID: WP_003404756.1
• Coordinates: 1021596..1022096 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JN148_RS04875
• Protein ID: WP_016719470.1
• Coordinates: 1021183..1021599 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS04855	1016970..1018241	-	1272	WP_003404747.1	PPE family protein	-
JN148_RS04860	1018256..1018555	-	300	WP_003404748.1	PE family protein	-
JN148_RS04865	1018999..1020780	+	1782	WP_003404749.1	BCCT family transporter	-
JN148_RS04870	1020780..1021034	+	255	WP_003898646.1	hypothetical protein	-
JN148_RS04875	1021183..1021599	+	417	WP_016719470.1	DUF1778 domain-containing protein	Antitoxin
JN148_RS04880	1021596..1022096	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
JN148_RS04890	1022409..1023728	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
JN148_RS04895	1024016..1024597	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
JN148_RS04900	1024597..1026249	+	1653	WP_003404761.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (45-136)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T189056 WP_003404756.1 NZ_CP069065:1021596-1022096 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T189056 NZ_CP090626:4164516-4164734 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15278.52 Da
• Isoelectric Point: 10.6899

>AT189056 WP_016719470.1 NZ_CP069065:1021183-1021599 [Mycobacterium tuberculosis]
MAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLTPEQDALIRRAAEAEGTDLTN
FTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 417 bp

>AT189056 NZ_CP090626:4164115-4164489 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2