Detailed information of TA system TA189046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 694595..695241
• Replicon: chromosome
• Accession: NZ_CP069065
• Organism: Mycobacterium tuberculosis strain N0091

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN148_RS03150
• Protein ID: WP_003403137.1
• Coordinates: 694595..695008 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN148_RS03155
• Protein ID: WP_003403139.1
• Coordinates: 695005..695241 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN148_RS03130	690678..692228	+	1551	WP_003403119.1	MlaD family protein	-
JN148_RS03135	692280..692672	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN148_RS03140	692669..692926	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN148_RS03145	693109..694344	-	1236	WP_003403128.1	ATP-binding protein	-
JN148_RS03150	694595..695008	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN148_RS03155	695005..695241	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN148_RS03160	695345..695851	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN148_RS03165	695965..696495	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN148_RS03170	696479..697174	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN148_RS03175	697297..697606	+	310	Protein_629	hypothetical protein	-
JN148_RS03180	697678..698628	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
JN148_RS03185	698869..699453	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
JN148_RS03190	699455..700165	+	711	Protein_632	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T189046 WP_003403137.1 NZ_CP069065:c695008-694595 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T189046 NZ_CP090623:c4881503-4881219 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT189046 WP_003403139.1 NZ_CP069065:c695241-695005 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT189046 NZ_CP090623:c4881735-4881493 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89