Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3091195..3091765 Replicon chromosome
Accession NZ_CP069064
Organism Mycobacterium tuberculosis strain N1202

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN149_RS14630 Protein ID WP_003414166.1
Coordinates 3091195..3091551 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN149_RS14635 Protein ID WP_003901465.1
Coordinates 3091535..3091765 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN149_RS14610 3086647..3088335 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN149_RS14615 3088339..3088665 - 327 WP_003414157.1 hypothetical protein -
JN149_RS14620 3088838..3089425 + 588 WP_031666959.1 DUF3558 family protein -
JN149_RS14625 3089444..3091093 + 1650 WP_031666960.1 CocE/NonD family hydrolase -
JN149_RS14630 3091195..3091551 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN149_RS14635 3091535..3091765 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN149_RS14640 3091808..3092851 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN149_RS14645 3092850..3093317 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN149_RS14650 3093493..3093747 - 255 WP_003917684.1 hypothetical protein -
JN149_RS14655 3093895..3094299 + 405 WP_003414181.1 hypothetical protein -
JN149_RS14660 3094296..3094487 + 192 WP_003414184.1 hypothetical protein -
JN149_RS14665 3094704..3094964 + 261 Protein_2896 transposase -
JN149_RS14670 3096074..3096331 + 258 WP_003899489.1 hypothetical protein -
JN149_RS14675 3096436..3096747 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189029 WP_003414166.1 NZ_CP069064:c3091551-3091195 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189029 NZ_CP090622:325848-326216 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189029 WP_003901465.1 NZ_CP069064:c3091765-3091535 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189029 NZ_CP090622:325630-325851 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References