Detailed information of TA system TA189028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3051612..3052299
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JN149_RS14410
• Protein ID: WP_003414064.1
• Coordinates: 3051904..3052299 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN149_RS14405
• Protein ID: WP_003414061.1
• Coordinates: 3051612..3051878 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS14375	3047251..3048153	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN149_RS14380	3048222..3048974	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN149_RS14390	3049218..3049493	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN149_RS14395	3049490..3051112	-	1623	WP_003910620.1	class I SAM-dependent DNA methyltransferase	-
JN149_RS14400	3051199..3051615	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
JN149_RS14405	3051612..3051878	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN149_RS14410	3051904..3052299	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN149_RS14415	3052296..3052565	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN149_RS14420	3052575..3053669	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN149_RS14425	3053666..3054085	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN149_RS14430	3054084..3054158	+	75	Protein_2849	hypothetical protein	-
JN149_RS14435	3054159..3054638	-	480	WP_003414073.1	dihydrofolate reductase	-
JN149_RS14440	3054709..3055509	-	801	WP_003911953.1	thymidylate synthase	-
JN149_RS14445	3055665..3056402	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T189028 WP_003414064.1 NZ_CP069064:c3052299-3051904 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T189028 NZ_CP090621:c14726-14310 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189028 WP_003414061.1 NZ_CP069064:c3051878-3051612 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189028 NZ_CP090621:c14953-14723 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64