Detailed information of TA system TA189023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2860109..2860818
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: JN149_RS13360
• Protein ID: WP_003413164.1
• Coordinates: 2860109..2860522 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN149_RS13365
• Protein ID: WP_003413167.1
• Coordinates: 2860561..2860818 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS13330	2855819..2856133	+	315	WP_009937839.1	hypothetical protein	-
JN149_RS13335	2856429..2857640	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN149_RS13340	2857767..2858426	+	660	WP_003900846.1	LppA family lipoprotein	-
JN149_RS13345	2858423..2859082	+	660	WP_003900847.1	LppA family lipoprotein	-
JN149_RS13350	2859079..2859741	+	663	WP_003900848.1	LppA family lipoprotein	-
JN149_RS13355	2859738..2860016	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN149_RS13360	2860109..2860522	+	414	WP_003413164.1	PIN domain nuclease	Toxin
JN149_RS13365	2860561..2860818	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN149_RS13370	2860815..2861192	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
JN149_RS13375	2861208..2861582	-	375	WP_003413177.1	hypothetical protein	-
JN149_RS13380	2861682..2862077	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN149_RS13385	2862074..2862319	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN149_RS13390	2862730..2863149	-	420	WP_003413190.1	A24 family peptidase	-
JN149_RS13395	2863161..2863970	-	810	WP_003910591.1	shikimate dehydrogenase	-
JN149_RS13400	2863967..2865220	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN149_RS13405	2865213..2865725	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T189023 WP_003413164.1 NZ_CP069064:2860109-2860522 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T189023 NZ_CP090616:c75876-75607 [Vibrio pelagius]
ATGAGTAGTAGTCAGCGCCTGCTCTCATGGACTGATGAGGCGTGGAACGACTATTTATACTGGCAAACTCAAGATAAGAA
AACACTAAAACGAGTCAACAAACTCATCAATGATGTCAAGCGGTCACCTTTTGAAGGTATTGGAAAGCCAGAACCGTTAA
AAGAGAATCTTTCGGGTTTTTGGTCTAGGCGAATTGATGAAACTAATCGATTGGTGTATGCCGTAGATGATCAAGCGATT
ACAGTTATTTCATGTCGCTACCATTACTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189023 WP_003413167.1 NZ_CP069064:2860561-2860818 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189023 NZ_CP090616:c76123-75869 [Vibrio pelagius]
ATGCGAATCGTATCTTTTACTGAAGCAAGGAACAGTCTTAAATCTGTACTAGATGGGGTGGTGGACGATGCTGATACCAC
GGTGATCACTCGTCGCGATTCCGAAGATGCTGTCGTTATGTCTTTAGACTATTACAACAGTTTGATGGAAACGGTGCACT
TGCTGCGCTCACCCAAGAATGCGGAGCATTTGAGTCGTTCTATTGAGCAATACCGTCATGGTAAGACAACGGAGCGAGAA
TTAATCGATGAGTAG

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5