Detailed information of TA system TA189022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2845566..2846206
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN149_RS13270
• Protein ID: WP_003412970.1
• Coordinates: 2845566..2845985 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN149_RS13275
• Protein ID: WP_003910588.1
• Coordinates: 2845982..2846206 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS13240	2841154..2841876	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN149_RS13245	2842393..2842620	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN149_RS13250	2842617..2843018	+	402	WP_003412963.1	PIN domain-containing protein	-
JN149_RS13255	2843053..2843973	-	921	WP_003412965.1	restriction endonuclease	-
JN149_RS13260	2844314..2844520	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN149_RS13265	2844618..2845565	+	948	WP_162097749.1	ERCC4 domain-containing protein	-
JN149_RS13270	2845566..2845985	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN149_RS13275	2845982..2846206	-	225	WP_003910588.1	hypothetical protein	Antitoxin
JN149_RS13280	2846237..2849080	-	2844	WP_003910589.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN149_RS13285	2849152..2849553	-	402	WP_003412981.1	hypothetical protein	-
JN149_RS13290	2849553..2850023	-	471	WP_003412985.1	transcription antitermination factor NusB	-
JN149_RS13295	2850026..2850589	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189022 WP_003412970.1 NZ_CP069064:c2845985-2845566 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189022 NZ_CP090615:c446372-446094 [Vibrio pelagius]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTTGAGTTTCTCTATGACTTCAACCCTGATGCTGC
AGAAAAAACAGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATAGAA
TCCGCGGACGATTGCTCATCATTCCTGAGATTTCGATGATTGTCTCTTACTGGATTGAGGGCGATATCATCCGAGTTATG
CGTGTACTACACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8021.99 Da
• Isoelectric Point: 4.0057

>AT189022 WP_003910588.1 NZ_CP069064:c2846206-2845982 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDVRSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189022 NZ_CP090615:c446653-446369 [Vibrio pelagius]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGAAC
TTTAAGTGATGCTTGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCGTTGAGCACCAAACGGCTAAGTCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGAGGTAAGCAATGA

Structures

Toxin

• AlphaFold DB: G0TQ08