Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2318134..2318770 Replicon chromosome
Accession NZ_CP069064
Organism Mycobacterium tuberculosis strain N1202

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag JN149_RS10790 Protein ID WP_003410654.1
Coordinates 2318360..2318770 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag JN149_RS10785 Protein ID WP_003410651.1
Coordinates 2318134..2318367 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN149_RS10770 2313591..2313983 + 393 WP_229303073.1 metal ABC transporter permease -
JN149_RS10775 2313984..2314388 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
JN149_RS10780 2314472..2318056 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
JN149_RS10785 2318134..2318367 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
JN149_RS10790 2318360..2318770 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
JN149_RS10795 2318754..2319845 + 1092 WP_003900454.1 precorrin-3B synthase -
JN149_RS10800 2319855..2320481 + 627 WP_003410658.1 precorrin-8X methylmutase -
JN149_RS10805 2320478..2322004 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
JN149_RS10810 2321950..2323173 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T189018 WP_003410654.1 NZ_CP069064:2318360-2318770 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T189018 NZ_CP090612:c293655-293553 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT189018 WP_003410651.1 NZ_CP069064:2318134-2318367 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT189018 NZ_CP090612:293515-293783 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References