Detailed information of TA system TA189014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2225044..2225942
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: JN149_RS10375
• Protein ID: WP_003410001.1
• Coordinates: 2225044..2225604 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: JN149_RS10380
• Protein ID: WP_003410003.1
• Coordinates: 2225601..2225942 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS10345	2220213..2220866	-	654	WP_003409976.1	cutinase Cfp21	-
JN149_RS10350	2220981..2221211	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JN149_RS10355	2221296..2222207	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN149_RS10360	2222316..2222915	+	600	WP_003409989.1	L-lysine exporter	-
JN149_RS10365	2223331..2223759	+	429	WP_003409992.1	cellulose-binding protein	-
JN149_RS10370	2223985..2224524	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN149_RS10375	2225044..2225604	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
JN149_RS10380	2225601..2225942	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
JN149_RS10385	2226028..2226285	+	258	WP_003410006.1	hypothetical protein	-
JN149_RS10390	2226186..2226521	-	336	WP_003410009.1	dehydrogenase	-
JN149_RS10395	2226610..2226954	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JN149_RS10400	2226948..2227196	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JN149_RS10405	2227296..2229611	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
JN149_RS10410	2229608..2229880	-	273	WP_003410017.1	DUF1490 family protein	-
JN149_RS10415	2229933..2230289	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T189014 WP_003410001.1 NZ_CP069064:c2225604-2225044 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T189014 NZ_CP090611:c3540374-3540078 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTTATCCGTATTCGCCCTCCAGAACGGCTTAACACAATCCTTTTACTGGTAG
ACGGCAAAGAATATGTGCTCATGACGCATGAGACTGAAACTGTTCCAGTTAACGCGCTGGGAACGAAATTTTGCGATGCC
AGCGCGCACCGAACGTTGATTAAAGGGGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT189014 WP_003410003.1 NZ_CP069064:c2225942-2225601 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT189014 NZ_CP090611:c3540610-3540377 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 6FKG

Antitoxin

• PDB: 6FKG
• AlphaFold DB: A0A7U4FAE4