Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2199667..2200370 Replicon chromosome
Accession NZ_CP069064
Organism Mycobacterium tuberculosis strain N1202

Toxin (Protein)


Gene name mazF Uniprot ID G0TLK7
Locus tag JN149_RS10180 Protein ID WP_003409778.1
Coordinates 2199667..2199996 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TLK8
Locus tag JN149_RS10185 Protein ID WP_003409780.1
Coordinates 2199993..2200370 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN149_RS10160 2196050..2197120 + 1071 WP_003899091.1 epoxide hydrolase EphB -
JN149_RS10165 2197117..2197632 + 516 WP_003409718.1 flavin reductase family protein -
JN149_RS10170 2197629..2198690 + 1062 WP_055375323.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
JN149_RS10175 2198687..2199457 + 771 WP_003409775.1 SDR family oxidoreductase -
JN149_RS10180 2199667..2199996 - 330 WP_003409778.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JN149_RS10185 2199993..2200370 - 378 WP_003409780.1 type II toxin-antitoxin system antitoxin MazE5 Antitoxin
JN149_RS10190 2200367..2200957 - 591 WP_003910477.1 SEC-C metal-binding domain-containing protein -
JN149_RS10195 2201012..2202376 + 1365 WP_003910478.1 HNH endonuclease signature motif containing protein -
JN149_RS10200 2202531..2202983 - 453 WP_003899095.1 lipoprotein -
JN149_RS10205 2203047..2203448 + 402 WP_003409869.1 hypothetical protein -
JN149_RS10210 2203441..2203623 - 183 WP_003409870.1 hypothetical protein -
JN149_RS10215 2203737..2204087 - 351 WP_003910480.1 hypothetical protein -
JN149_RS10220 2204098..2205000 - 903 WP_003409874.1 hypothetical protein -
JN149_RS10225 2205021..2205212 - 192 WP_003409876.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-103)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11787.82 Da        Isoelectric Point: 8.5361

>T189010 WP_003409778.1 NZ_CP069064:c2199996-2199667 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Download         Length: 330 bp

>T189010 NZ_CP090611:2240286-2240393 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13560.07 Da        Isoelectric Point: 5.1519

>AT189010 WP_003409780.1 NZ_CP069064:c2200370-2199993 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Download         Length: 378 bp

>AT189010 NZ_CP090611:c2240236-2240173 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TLK7

Antitoxin

Source ID Structure
AlphaFold DB G0TLK8

References