Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1689652..1690268 Replicon chromosome
Accession NZ_CP069064
Organism Mycobacterium tuberculosis strain N1202

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JN149_RS07955 Protein ID WP_003407593.1
Coordinates 1689951..1690268 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A109SWY9
Locus tag JN149_RS07950 Protein ID WP_003910377.1
Coordinates 1689652..1689954 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN149_RS07940 1685538..1687385 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JN149_RS07945 1687386..1689638 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JN149_RS07950 1689652..1689954 + 303 WP_003910377.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JN149_RS07955 1689951..1690268 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JN149_RS07960 1690265..1691269 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JN149_RS07965 1691322..1692611 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
JN149_RS07970 1692684..1693301 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
JN149_RS07975 1693515..1693706 - 192 WP_003407609.1 dodecin family protein -
JN149_RS07980 1693788..1694186 + 399 WP_003900351.1 hypothetical protein -
JN149_RS07985 1694231..1695261 + 1031 Protein_1575 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T189006 WP_003407593.1 NZ_CP069064:1689951-1690268 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T189006 NZ_CP090611:1845928-1846179 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11203.45 Da        Isoelectric Point: 8.5236

>AT189006 WP_003910377.1 NZ_CP069064:1689652-1689954 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARAWRNARQQRSAQ

Download         Length: 303 bp

>AT189006 NZ_CP090611:1846176-1846430 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A109SWY9

References