Detailed information of TA system TA189002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1382819..1383507
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: JN149_RS06610
• Protein ID: WP_003406304.1
• Coordinates: 1383076..1383507 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: JN149_RS06605
• Protein ID: WP_003406302.1
• Coordinates: 1382819..1383079 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS06585	1378396..1379220	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
JN149_RS06590	1379225..1380406	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
JN149_RS06595	1380483..1381583	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
JN149_RS06600	1381754..1382743	+	990	WP_003910306.1	malate dehydrogenase	-
JN149_RS06605	1382819..1383079	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
JN149_RS06610	1383076..1383507	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN149_RS06615	1383530..1385216	-	1687	Protein_1308	PE family protein	-
JN149_RS06620	1385396..1386256	+	861	WP_003406306.1	glycine betaine ABC transporter substrate-binding protein	-
JN149_RS06625	1386337..1387167	-	831	WP_003910309.1	SDR family oxidoreductase	-
JN149_RS06630	1387224..1387517	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
JN149_RS06635	1387514..1387783	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189002 WP_003406304.1 NZ_CP069064:1383076-1383507 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189002 NZ_CP090611:320215-320592 [Escherichia coli]
ATGACCATTCAGTTTATCTCTGCGGAGGAGATAATTTATTTCCACGATAAGTTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTACGAGTATGAAGGCGTTTCCGATCTTT
GGCTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATTTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCCTCATACTTGCTGCAAACCCAGAGTTTGTGGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACACGGAACCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189002 WP_003406302.1 NZ_CP069064:1382819-1383079 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189002 NZ_CP090611:319997-320218 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGACAGAACCTTGCATTGGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCCGCTGTGATTATTAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CGGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTATGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6