Detailed information of TA system TA189001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1236605..1237173
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN149_RS05945
• Protein ID: WP_003405865.1
• Coordinates: 1236799..1237173 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN149_RS05940
• Protein ID: WP_003405863.1
• Coordinates: 1236605..1236802 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS05920	1232646..1233284	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN149_RS05925	1233374..1234381	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN149_RS05930	1234398..1235381	-	984	WP_003898731.1	hypothetical protein	-
JN149_RS05935	1235444..1236517	+	1074	WP_003405860.1	redox-regulated ATPase YchF	-
JN149_RS05940	1236605..1236802	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN149_RS05945	1236799..1237173	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN149_RS05950	1237376..1238074	+	699	WP_003898733.1	hypothetical protein	-
JN149_RS05955	1238192..1238377	+	186	WP_003901093.1	hypothetical protein	-
JN149_RS05960	1238304..1238579	-	276	WP_003405867.1	hypothetical protein	-
JN149_RS05965	1238822..1239145	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN149_RS05970	1239160..1239866	-	707	Protein_1178	guanylate cyclase	-
JN149_RS05975	1239888..1240097	+	210	WP_003911400.1	hypothetical protein	-
JN149_RS05980	1240049..1240819	-	771	Protein_1180	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189001 WP_003405865.1 NZ_CP069064:1236799-1237173 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189001 NZ_CP090611:292713-292988 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTGCGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189001 WP_003405863.1 NZ_CP069064:1236605-1236802 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189001 NZ_CP090611:292985-293344 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07