Detailed information of TA system TA188983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 359673..360244
• Replicon: chromosome
• Accession: NZ_CP069064
• Organism: Mycobacterium tuberculosis strain N1202

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: JN149_RS01585
• Protein ID: WP_003401560.1
• Coordinates: 359673..359975 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: JN149_RS01590
• Protein ID: WP_003401563.1
• Coordinates: 360023..360244 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN149_RS01565	355097..355900	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
JN149_RS01570	355910..357307	-	1398	WP_013988728.1	sulfatase	-
JN149_RS01575	357486..359306	+	1821	WP_003401546.1	PE family protein	-
JN149_RS01580	359449..359676	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
JN149_RS01585	359673..359975	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
JN149_RS01590	360023..360244	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
JN149_RS01595	360241..360666	+	426	WP_003401566.1	PIN domain nuclease	-
JN149_RS01600	360802..361434	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
JN149_RS01605	361431..362339	+	909	WP_003900117.1	protochlorophyllide reductase	-
JN149_RS01610	362347..362937	-	591	WP_229298012.1	hypothetical protein	-
JN149_RS01615	362930..362980	-	51	Protein_320	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T188983 WP_003401560.1 NZ_CP069064:359673-359975 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T188983 NZ_CP090597:4834863-4835150 [Pectobacterium aroidearum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAATTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCATCAGGCTTTCGTTTGGTTTATGAAGTCATTGACGATGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAACGCAGTGGCGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT188983 WP_003401563.1 NZ_CP069064:360023-360244 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT188983 NZ_CP090597:4834615-4834866 [Pectobacterium aroidearum]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAATTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGACGGTCTACCTGTCGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCTTATGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48