Detailed information of TA system TA188978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3767880..3768409
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN150_RS17675
• Protein ID: WP_003417760.1
• Coordinates: 3768152..3768409 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN150_RS17670
• Protein ID: WP_003417757.1
• Coordinates: 3767880..3768155 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS17635	3764453..3765142	-	690	WP_003917774.1	BBE domain-containing protein	-
JN150_RS17640	3765329..3765700	-	372	WP_003417739.1	FAD-binding protein	-
JN150_RS17645	3765598..3765816	-	219	WP_157132559.1	hypothetical protein	-
JN150_RS17650	3765843..3766103	-	261	WP_003417741.1	hypothetical protein	-
JN150_RS17655	3766218..3766607	+	390	WP_050069136.1	DUF732 domain-containing protein	-
JN150_RS17660	3766621..3766914	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN150_RS17665	3766911..3767756	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN150_RS17670	3767880..3768155	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN150_RS17675	3768152..3768409	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN150_RS17680	3768451..3769641	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN150_RS17685	3769758..3770126	+	369	WP_003417765.1	FHA domain-containing protein	-
JN150_RS17690	3770123..3770674	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN150_RS17695	3770681..3771262	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN150_RS17700	3771243..3771611	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN150_RS17705	3771592..3771981	-	390	WP_050069135.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T188978 WP_003417760.1 NZ_CP069063:3768152-3768409 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T188978 NZ_CP090597:760215-760589 [Pectobacterium aroidearum]
ATGCAACTGCTTTCATTGATTGTGCATGGCCTATTAGTATTGCTCATTCTTCTGGCGCCGTGGCCGGACGGCTATGCATG
GCTGTGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTATCCGTAGCCAAAGGAATATCAAATCCCGGCAGGGGGAAA
TCGTTTTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAGGAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAAT
GGCGTATTGCTGTCATTGCAGGCAGTGAACGGTAAAGACAGACAACAACTGTGGCTGGCATCGGACAGCATGGGCGATGA
CGAATGGCGTCATCTGCGTCAGCTTCTTTTGCAGCAGAAAAACTGGGCGAGATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT188978 WP_003417757.1 NZ_CP069063:3767880-3768155 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT188978 NZ_CP090597:759929-760195 [Pectobacterium aroidearum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGGGGTATGCGTGAGTTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTACGACACACTCAGTGACAGCGATAAGCGCACCTTTGTGCGCCTGTTACAAAGCGATGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94