Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3130981..3131585 Replicon chromosome
Accession NZ_CP069063
Organism Mycobacterium tuberculosis strain N3913

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN150_RS14865 Protein ID WP_003414492.1
Coordinates 3130981..3131373 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN150_RS14870 Protein ID WP_003414495.1
Coordinates 3131370..3131585 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN150_RS14835 3126131..3126919 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JN150_RS14840 3127253..3127798 - 546 WP_003904931.1 DUF1802 family protein -
JN150_RS14845 3128070..3128954 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN150_RS14850 3128957..3129844 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN150_RS14855 3130149..3130694 - 546 WP_031705426.1 DUF1802 family protein -
JN150_RS14860 3130691..3130960 - 270 WP_003414489.1 DUF2277 family protein -
JN150_RS14865 3130981..3131373 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN150_RS14870 3131370..3131585 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN150_RS14875 3131632..3132381 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN150_RS14880 3132460..3133542 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN150_RS14885 3133535..3134845 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JN150_RS14890 3134848..3135675 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN150_RS14895 3135672..3136583 - 912 WP_050069471.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T188973 WP_003414492.1 NZ_CP069063:c3131373-3130981 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T188973 NZ_CP090597:168691-168966 [Pectobacterium aroidearum]
ATGCAGGGAAAAATAGCCAGTCTACGAAAAAAGCAGCGAGAAACGCTGAGCCAAATTTTCAAAACACCCGTGCTTTCAGG
TGTTAAATGGTCAAATGTTGAGTCACTCATCACTGCGTTAGGTGGTGAAATTAAGGAAGGTAGCGGGTCACGGGTGCGAT
TTTTATTGAATGGGAGTATCGCCCGGTTCCATCGTCCTCACCCGTCACCGGATACTGATAAAGGCGCATTGGTTAACCTG
CGTGAATGGTTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT188973 WP_003414495.1 NZ_CP069063:c3131585-3131370 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT188973 NZ_CP090597:168963-169325 [Pectobacterium aroidearum]
ATGACTAAAGCACTTAACACACCAAATACGATGGTTGTTTCCGGCCAACCGGCCATTATCAGCTATGTACCGGAAATCGG
TATGTTTCGCGGTAAGTTTATTGGCTTATCTGGCTACTGTGATTTTGTCGCTGACAGTATTAATGGGCTGAGAAACGAGG
GCGAAATTTCGCTACGTGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAACGTGAAGAGAAGGTGAAA
ACGTTCACTCTCCGTTATCCTGAATCATTCGGAGAACGACTGACTATTGCGGCGGCTGAACGTCAAATTTCAGTGAATAC
GTTTATTGTCGAAACGCTTAATGAACGTATGAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References