Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3105089..3105659 Replicon chromosome
Accession NZ_CP069063
Organism Mycobacterium tuberculosis strain N3913

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN150_RS14720 Protein ID WP_003414166.1
Coordinates 3105089..3105445 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN150_RS14725 Protein ID WP_003901465.1
Coordinates 3105429..3105659 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN150_RS14700 3100541..3102229 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN150_RS14705 3102233..3102559 - 327 WP_003414157.1 hypothetical protein -
JN150_RS14710 3102732..3103319 + 588 WP_003914429.1 DUF3558 family protein -
JN150_RS14715 3103338..3104987 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN150_RS14720 3105089..3105445 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN150_RS14725 3105429..3105659 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN150_RS14730 3105702..3106745 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
JN150_RS14735 3106744..3107211 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN150_RS14740 3107387..3107641 - 255 WP_003917684.1 hypothetical protein -
JN150_RS14745 3107789..3108193 + 405 WP_003414181.1 hypothetical protein -
JN150_RS14750 3108190..3108381 + 192 WP_003414184.1 hypothetical protein -
JN150_RS14755 3108598..3108858 + 261 Protein_2911 transposase -
JN150_RS14760 3109968..3110225 + 258 WP_003899489.1 hypothetical protein -
JN150_RS14765 3110330..3110641 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T188971 WP_003414166.1 NZ_CP069063:c3105445-3105089 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T188971 NZ_CP090596:4916718-4917005 [Pectobacterium aroidearum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAATTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCATCAGGCTTTCGTTTGGTTTATGAAGTCATTGACGATGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAACGCAGTGGCGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT188971 WP_003901465.1 NZ_CP069063:c3105659-3105429 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT188971 NZ_CP090596:4916470-4916721 [Pectobacterium aroidearum]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAATTGAAAAAGAACCCGATGGCGACGGTCAATGCTGG
CGACGGTCTACCTGTCGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCGCTGGTTGACGTGGATTTGGAT
AGTTTCTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References