Detailed information of TA system TA188969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3065092..3065771
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN150_RS14490
• Protein ID: WP_003414059.1
• Coordinates: 3065092..3065508 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN150_RS14495
• Protein ID: WP_003414061.1
• Coordinates: 3065505..3065771 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS14465	3061144..3062046	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN150_RS14470	3062115..3062867	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN150_RS14480	3063111..3063386	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN150_RS14485	3063383..3065005	-	1623	WP_050069575.1	class I SAM-dependent DNA methyltransferase	-
JN150_RS14490	3065092..3065508	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN150_RS14495	3065505..3065771	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN150_RS14500	3065797..3066192	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN150_RS14505	3066189..3066458	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN150_RS14510	3066468..3067562	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN150_RS14515	3067559..3067978	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN150_RS14520	3067977..3068051	+	75	Protein_2864	hypothetical protein	-
JN150_RS14525	3068052..3068531	-	480	WP_003414073.1	dihydrofolate reductase	-
JN150_RS14530	3068602..3069402	-	801	WP_003911953.1	thymidylate synthase	-
JN150_RS14535	3069558..3070295	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T188969 WP_003414059.1 NZ_CP069063:c3065508-3065092 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T188969 NZ_CP090596:c3182397-3181993 [Pectobacterium aroidearum]
ATGCTGAAATATCTACTTGATACCAATATCTGTATTTACACTATCAAGAACAAACCGCAAGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGCTGATGGAACTGATCTATGGTGCGGAGAAATCGATAAATCCAG
AAAAGAATCTCGCCGTAATTGAAGGGTTTTCTGCGCGTCTGGAAGTGCAAAATTATGGTTTTGGCGCCGCAGTGCATACT
GGGCAAATTCGTGCGGAACTGGCAAGACAAGGAACCCCCATCGGCCCGTATGATGCCATGCTAGCGGGTCATGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAATTTGAGCGTGTGCCTGGATTGCGCGTAGAAAACTGGGTCGGTA
GATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT188969 WP_003414061.1 NZ_CP069063:c3065771-3065505 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT188969 NZ_CP090596:c3182627-3182397 [Pectobacterium aroidearum]
ATGGAAAAAACTACAGTATTTAAAAGCAATCGCAGCCAGGCGGTACGGCTCCCTAAAGCCGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGTCGAACACGTATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGACGGGGTAACGCCTGACTTTATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTTTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64