Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2875461..2876098 Replicon chromosome
Accession NZ_CP069063
Organism Mycobacterium tuberculosis strain N3913

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN150_RS13470 Protein ID WP_003413180.1
Coordinates 2875461..2875856 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN150_RS13475 Protein ID WP_003413183.1
Coordinates 2875853..2876098 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN150_RS13430 2871546..2872205 + 660 WP_050069193.1 LppA family lipoprotein -
JN150_RS13435 2872202..2872861 + 660 WP_003900847.1 LppA family lipoprotein -
JN150_RS13440 2872858..2873493 + 636 WP_031685723.1 LppA family lipoprotein -
JN150_RS13445 2873517..2873795 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN150_RS13450 2873888..2874301 + 414 WP_003413164.1 PIN domain nuclease -
JN150_RS13455 2874340..2874597 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN150_RS13460 2874594..2874971 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN150_RS13465 2874987..2875361 - 375 WP_003413177.1 hypothetical protein -
JN150_RS13470 2875461..2875856 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN150_RS13475 2875853..2876098 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN150_RS13480 2876509..2876928 - 420 WP_003413190.1 A24 family peptidase -
JN150_RS13485 2876940..2877749 - 810 WP_003413193.1 shikimate dehydrogenase -
JN150_RS13490 2877746..2878999 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN150_RS13495 2878992..2879504 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T188967 WP_003413180.1 NZ_CP069063:c2875856-2875461 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T188967 NZ_CP090596:c1013657-1013517 [Pectobacterium aroidearum]
ATGACAACTGAACTATCTAGCATTCTGATTGAGGTGATTGGTGAGACTATCGCGGATCTTGGCGAGTATGCCGATGAGAT
TAAGGATGCTATTAATCTTATTTTTGGGGATTTGAAAGGTCATTCACTGCTACTCAGCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT188967 WP_003413183.1 NZ_CP069063:c2876098-2875853 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT188967 NZ_CP090596:c1013856-1013647 [Pectobacterium aroidearum]
ATGAAACGCCGGATTAGCATTGCCGTGGATAAAAACGATAATCAGATTCCCAATGCTACCGGGATACCTATTTCTGGTCT
GGTGAATGACGTCATAGGCACAGAAGCCTACCGCATCAAAACTGAGGCGTGGAAGAAGGAAAACCGCGAAGGGATGGGAG
AAGTCGCCCAATTTATCGCACAGTATGGTTCCTTTGCGGATGACAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References