Detailed information of TA system TA188964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2859345..2859985
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JN150_RS13360
• Protein ID: WP_244734975.1
• Coordinates: 2859345..2859764 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN150_RS13365
• Protein ID: WP_003412975.1
• Coordinates: 2859761..2859985 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS13330	2854930..2855652	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN150_RS13335	2856169..2856396	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN150_RS13340	2856393..2856794	+	402	WP_003412963.1	PIN domain-containing protein	-
JN150_RS13345	2856829..2857749	-	921	WP_003412965.1	restriction endonuclease	-
JN150_RS13350	2858090..2858296	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN150_RS13355	2858394..2859344	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN150_RS13360	2859345..2859764	-	420	WP_244734975.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN150_RS13365	2859761..2859985	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN150_RS13370	2860016..2862859	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN150_RS13375	2862931..2863332	-	402	WP_003412981.1	hypothetical protein	-
JN150_RS13380	2863332..2863802	-	471	WP_003412985.1	transcription antitermination factor NusB	-
JN150_RS13385	2863805..2864368	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14746.73 Da
• Isoelectric Point: 6.7519

>T188964 WP_244734975.1 NZ_CP069063:c2859764-2859345 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSLMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T188964 NZ_CP090596:c515245-514991 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAAAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAGGGAAAAGGAAAACCAGAGCCACTTAAACATAATCTTGCTG
GTTTCTGGTCGCGCCGTATCACCGAAGAACATCGTCTGGTTTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT188964 WP_003412975.1 NZ_CP069063:c2859985-2859761 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT188964 NZ_CP090596:c515493-515242 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTCATGTCTTTGGAAGAATATAACTCTCTAGAGGAGACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAAGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: G0TQ09