Detailed information of TA system TA188956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2240358..2241256
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: JN150_RS10470
• Protein ID: WP_003410001.1
• Coordinates: 2240358..2240918 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: JN150_RS10475
• Protein ID: WP_003410003.1
• Coordinates: 2240915..2241256 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS10440	2235527..2236180	-	654	WP_003409976.1	cutinase Cfp21	-
JN150_RS10445	2236295..2236525	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JN150_RS10450	2236610..2237521	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN150_RS10455	2237630..2238229	+	600	WP_003409989.1	L-lysine exporter	-
JN150_RS10460	2238645..2239073	+	429	WP_003409992.1	cellulose-binding protein	-
JN150_RS10465	2239299..2239838	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN150_RS10470	2240358..2240918	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
JN150_RS10475	2240915..2241256	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
JN150_RS10480	2241342..2241599	+	258	WP_003410006.1	hypothetical protein	-
JN150_RS10485	2241500..2241835	-	336	WP_003410009.1	dehydrogenase	-
JN150_RS10490	2241924..2242268	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JN150_RS10495	2242262..2242510	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JN150_RS10500	2242610..2244925	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
JN150_RS10505	2244922..2245194	-	273	WP_003410017.1	DUF1490 family protein	-
JN150_RS10510	2245247..2245603	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T188956 WP_003410001.1 NZ_CP069063:c2240918-2240358 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T188956 NZ_CP090595:c1227062-1226859 [Pectobacterium aroidearum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCAGAACTGACGATGAACAAAC
TTTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT188956 WP_003410003.1 NZ_CP069063:c2241256-2240915 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT188956 NZ_CP090595:c1227484-1227116 [Pectobacterium aroidearum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGGATTAATGATGCTGAACTGCGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• PDB: 6FKG

Antitoxin

• PDB: 6FKG
• AlphaFold DB: A0A7U4FAE4