Detailed information of TA system TA188952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197774..2198477
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN150_RS10180
• Protein ID: WP_003409778.1
• Coordinates: 2197774..2198103 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN150_RS10185
• Protein ID: WP_003409780.1
• Coordinates: 2198100..2198477 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS10160	2194158..2195228	+	1071	WP_050069306.1	epoxide hydrolase EphB	-
JN150_RS10165	2195225..2195739	+	515	Protein_2006	flavin reductase family protein	-
JN150_RS10170	2195736..2196797	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN150_RS10175	2196794..2197564	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN150_RS10180	2197774..2198103	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN150_RS10185	2198100..2198477	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN150_RS10190	2198474..2199064	-	591	WP_050069305.1	SEC-C metal-binding domain-containing protein	-
JN150_RS10195	2199119..2200483	+	1365	WP_193019714.1	HNH endonuclease signature motif containing protein	-
JN150_RS10200	2200638..2201090	-	453	WP_003899095.1	lipoprotein	-
JN150_RS10205	2201154..2201555	+	402	WP_003409869.1	hypothetical protein	-
JN150_RS10210	2201548..2201730	-	183	WP_003409870.1	hypothetical protein	-
JN150_RS10215	2201844..2202194	-	351	WP_003409871.1	hypothetical protein	-
JN150_RS10220	2202205..2203107	-	903	WP_003409874.1	hypothetical protein	-
JN150_RS10225	2203128..2203319	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T188952 WP_003409778.1 NZ_CP069063:c2198103-2197774 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T188952 NZ_CP090595:368942-369229 [Pectobacterium aroidearum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCAGAGAACAATTCAA
AAAAAAGCTAGTTGAGCGTTTAGAAAATCCTCGTGTTCCTTCAGCCCAGCTCCATGGTCGTAAAGACCAGTATAAAATTA
AACTGAAGTCTGCTGGTTACAGGCTGGTGTATTTGGTTCAGGATGAGACCATTACGGTGATGGTCATGGGGGTTGGAAAG
CGCGAAGGTAGTCAGGTTTATTCTGATACTAAAGGTCGCTCTGCTTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT188952 WP_003409780.1 NZ_CP069063:c2198477-2198100 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT188952 NZ_CP090595:368698-368952 [Pectobacterium aroidearum]
ATGGCTTTCAATATCCTAACTAACACAGCGGCCAGTATCACTGAACTGAAACGCGACCCAATGGGGACAGTACGGGCTGC
TGAAGGTGAGACTATCGCTATACTGAACCGGAATGAGCCTGCGTTCTATTGTGTGCCTCCTGCCATGTTCGCTTATTTAA
TGGAATTGGCTGAAGATGCAGAGTTGGGGCGTACTGTTGATGATCGTATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8