Detailed information of TA system TA188949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1770888..1771501
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: JN150_RS08300
• Protein ID: WP_003407786.1
• Coordinates: 1771097..1771501 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: JN150_RS08295
• Protein ID: WP_009935474.1
• Coordinates: 1770888..1771091 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS08280	1766152..1769055	+	2904	WP_050069233.1	MMPL family transporter	-
JN150_RS08285	1769065..1769511	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
JN150_RS08290	1769546..1770835	+	1290	WP_003407781.1	threonine ammonia-lyase	-
JN150_RS08295	1770888..1771091	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN150_RS08300	1771097..1771501	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
JN150_RS08305	1771518..1773260	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
JN150_RS08310	1773253..1775550	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T188949 WP_003407786.1 NZ_CP069063:1771097-1771501 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T188949 NZ_CP090595:c26631-26245 [Pectobacterium aroidearum]
ATGGAGGTTTACAGATTGGCATGGGAAATAGTCACACGGGCGCTATTCGATACGTGGTTTGATGAGCAAACCGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGGTATACTCGAAGAAGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCACAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAT
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGAAAAATTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACATTTAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT188949 WP_009935474.1 NZ_CP069063:1770888-1771091 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT188949 NZ_CP090595:c26258-25959 [Pectobacterium aroidearum]
ATGGCGACATTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCATTAGCTCTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAGAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACAGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46