Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1577556..1578211 Replicon chromosome
Accession NZ_CP069063
Organism Mycobacterium tuberculosis strain N3913

Toxin (Protein)


Gene name vapC Uniprot ID A0A7U4FAR1
Locus tag JN150_RS07450 Protein ID WP_003407268.1
Coordinates 1577556..1577957 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag JN150_RS07455 Protein ID WP_003407272.1
Coordinates 1577954..1578211 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN150_RS07435 1573103..1574488 - 1386 WP_050069436.1 cytochrome P450 -
JN150_RS07440 1574566..1575600 + 1035 WP_003407264.1 AraC family transcriptional regulator -
JN150_RS07445 1575646..1577301 - 1656 Protein_1465 PE family protein -
JN150_RS07450 1577556..1577957 - 402 WP_003407268.1 type II toxin-antitoxin system VapC family toxin Toxin
JN150_RS07455 1577954..1578211 - 258 WP_003407272.1 CopG family transcriptional regulator Antitoxin
JN150_RS07460 1578294..1579253 - 960 WP_050069140.1 alpha/beta hydrolase -
JN150_RS07465 1579278..1580240 - 963 WP_003407279.1 alpha/beta hydrolase -
JN150_RS07470 1580239..1580976 + 738 WP_031647000.1 lysoplasmalogenase -
JN150_RS07475 1581057..1583024 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14894.39 Da        Isoelectric Point: 11.6897

>T188947 WP_003407268.1 NZ_CP069063:c1577957-1577556 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQGLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T188947 NZ_CP090594:c4435763-4435476 [Pectobacterium aroidearum]
ATGATCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAATTAGGGCATCCTGTCAGGGAACAATTCAA
AAAAAAGCTAGTTGAGCGTTTAGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGTCGTAAAGACCAGTATAAAATTA
AACTGAAGTCTGCTGGCTACAGACTGGTATATTTGGTTCAGGATGAGACCATTACGGTGATGGTCATGGGGGGTGGAAAG
CGCGAAGGTAGTCAGGTTTATTCTGATACTAAAGGTCGCTCTGCTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT188947 WP_003407272.1 NZ_CP069063:c1578211-1577954 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT188947 NZ_CP090594:c4436007-4435753 [Pectobacterium aroidearum]
ATGGCTTTCAATATCCTAACTAACACAGCGGCCAGCATCACTGAACTGAAACGCGACCCAATGGGGACAGTACGGGCTGC
TGAAGGTGAGACTATCGCTATACTGAACCGGAATGAGCCTGCGTTCTATTGTGTGCCTCCTGCCATGTTCGCTTATTTAA
TGGAATTGGCTGAAGATGCAGAGCTGGGGCGTACTGTTGATGATCGTATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGATCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FAR1

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References