Detailed information of TA system TA188940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1026878..1027851
• Replicon: chromosome
• Accession: NZ_CP069063
• Organism: Mycobacterium tuberculosis strain N3913

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: O05911
• Locus tag: JN150_RS04870
• Protein ID: WP_003404756.1
• Coordinates: 1027351..1027851 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JN150_RS04865
• Protein ID: WP_050069935.1
• Coordinates: 1026878..1027354 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN150_RS04845	1022725..1023996	-	1272	WP_003404747.1	PPE family protein	-
JN150_RS04850	1024011..1024310	-	300	WP_003404748.1	PE family protein	-
JN150_RS04855	1024754..1026535	+	1782	WP_003404749.1	BCCT family transporter	-
JN150_RS04860	1026535..1026789	+	255	WP_003898646.1	hypothetical protein	-
JN150_RS04865	1026878..1027354	+	477	WP_050069935.1	DUF1778 domain-containing protein	Antitoxin
JN150_RS04870	1027351..1027851	+	501	WP_003404756.1	GNAT family N-acetyltransferase	Toxin
JN150_RS04880	1028164..1029483	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
JN150_RS04885	1029771..1030352	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
JN150_RS04890	1030352..1032004	+	1653	WP_003404761.1	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1026532..1072443	45911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T188940 WP_003404756.1 NZ_CP069063:1027351-1027851 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T188940 NZ_CP090594:3534319-3534522 [Pectobacterium aroidearum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTTGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCAGAACTGACGATGAACAAAC
TTTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17417.03 Da
• Isoelectric Point: 11.4531

>AT188940 WP_050069935.1 NZ_CP069063:1026878-1027354 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKRERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT188940 NZ_CP090594:3533897-3534265 [Pectobacterium aroidearum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAACTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGGATTAATGATGCTGAACTGCGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BSZ2