Detailed information of TA system TA188918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3777606..3778135
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN151_RS17705
• Protein ID: WP_003417760.1
• Coordinates: 3777878..3778135 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN151_RS17700
• Protein ID: WP_003417757.1
• Coordinates: 3777606..3777881 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS17665	3774179..3774868	-	690	WP_003917774.1	BBE domain-containing protein	-
JN151_RS17670	3775055..3775426	-	372	WP_003417739.1	FAD-binding protein	-
JN151_RS17675	3775324..3775542	-	219	WP_157132559.1	hypothetical protein	-
JN151_RS17680	3775569..3775829	-	261	WP_003417741.1	hypothetical protein	-
JN151_RS17685	3775944..3776333	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN151_RS17690	3776347..3776640	-	294	WP_031663850.1	DUF3017 domain-containing protein	-
JN151_RS17695	3776637..3777482	-	846	WP_003905054.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN151_RS17700	3777606..3777881	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN151_RS17705	3777878..3778135	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN151_RS17710	3778177..3779367	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN151_RS17715	3779484..3779852	+	369	WP_003417765.1	FHA domain-containing protein	-
JN151_RS17720	3779849..3780400	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN151_RS17725	3780407..3780988	-	582	WP_031662192.1	ATP/GTP-binding protein	-
JN151_RS17730	3780969..3781337	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN151_RS17735	3781315..3781707	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T188918 WP_003417760.1 NZ_CP069062:3777878-3778135 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T188918 NZ_CP090592:c3066057-3065653 [Pectobacterium aroidearum]
ATGCTGAAATATCTACTTGATACCAATATCTGTATTTACACTATCAAGAACAAACCGCAAGAAGTCAGGGACGCTTTCTA
CCGCCATTACGGGCAGTTTGCTATCAGTTCTATCACGCTGATGGAACTGATCTATGGTGCGGAGAAATCGATAAATCCAG
AAAAGAATCTCGCCGTAATTGAAGGGTTTTCTGCGCGTCTGGAAGTGCAAAATTATGGTTTTGGCGCCGCAGTGCATACT
GGGCAAATTCGTGCGGAACTGGCAAGACAAGGAACCCCCATCGGCCCGTATGATGCCATGCTAGCGGGTCATGCTCGGTC
AGCAGGTTTGATACTGGTAACGAACAATGTGCGTGAATTTGAGCGTGTGCCTGGATTGCGCGTAGAAAACTGGGTCGGTA
GATAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT188918 WP_003417757.1 NZ_CP069062:3777606-3777881 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT188918 NZ_CP090592:c3066287-3066057 [Pectobacterium aroidearum]
ATGGAAAAAACTACAGTATTTAAAAGCAATCGCAGCCAGGCGGTACGGCTCCCTAAAGCCGTTGCACTGCCCGATGATGT
GAAGCAGGTTGATATCGTTGCGATTGGTCGAACACGTATCATTACTCCGGCAGGCGAAAGCTGGGACAGTTGGTTCGAAG
AGGACGGGGTAACGCCTGACTTTATGATCACCAGAGAGCAACCCGATGATCAGATCAGGGAAGATTTTTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94