Detailed information of TA system TA188917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3708584..3709258
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: JN151_RS17485
• Protein ID: WP_003417282.1
• Coordinates: 3708584..3709012 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: JN151_RS17490
• Protein ID: WP_003417286.1
• Coordinates: 3709016..3709258 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS17460	3704406..3704807	-	402	WP_003417264.1	cytidine deaminase	-
JN151_RS17465	3705044..3705382	+	339	WP_003904248.1	succinate dehydrogenase, cytochrome b556 subunit	-
JN151_RS17470	3705379..3705813	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
JN151_RS17475	3705942..3707714	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
JN151_RS17480	3707714..3708505	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
JN151_RS17485	3708584..3709012	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN151_RS17490	3709016..3709258	-	243	WP_003417286.1	CopG family transcriptional regulator	Antitoxin
JN151_RS17495	3709380..3709994	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
JN151_RS17500	3709991..3710656	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
JN151_RS17505	3710657..3711190	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
JN151_RS17510	3711187..3711387	-	201	Protein_3460	4a-hydroxytetrahydrobiopterin dehydratase	-
JN151_RS17515	3711438..3712699	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-
JN151_RS17520	3712740..3712919	-	180	Protein_3462	4a-hydroxytetrahydrobiopterin dehydratase	-
JN151_RS17525	3713016..3714020	-	1005	WP_003914475.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T188917 WP_003417282.1 NZ_CP069062:c3709012-3708584 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T188917 NZ_CP090592:c1234871-1234668 [Pectobacterium aroidearum]
ATGAAAAAAATCGACTATTTGATGCGTTTGCGTAAATGCACAACCATTGACACTCTCGAACGCGTTATTGAAAAAAACAA
GTATGAACTCTCTAATGATGAACTGGAGATGTTCTTTTCCGCAGCCGATCATCGTCTGGCAGAACTGACGATGAACAAAC
TTTACGACAAAGTTCCTACCGCAGTATGGCGATACGTCCGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT188917 WP_003417286.1 NZ_CP069062:c3709258-3709016 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT188917 NZ_CP090592:c1235293-1234925 [Pectobacterium aroidearum]
ATGGATGAGTACACACCAAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAGAATCTGTGTGACGAAAGCATAGC
AACGTTAGGCGATAGCAGCCACGGCTGGGTCAATGATCCAACATCTGCGATCAATCTCCAATTAAATGAACTTATTGAGC
ATATCGCTACGTTTATTCTCACATTTAAAATAAAATACCCTAACGAAAGTGAGCTTTCAGAGCAGGTTGAAAAGTATTTG
GATGATACTTACGTCTTGTTTAGCAACTATGGGATTAATGATGCTGAACTGCGGCGCTGGCAGAAGTCCAAAGCAAAATT
ATTCGGAATGTTCTCAGGGGAGAACGTCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59