Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3107644..3108214 Replicon chromosome
Accession NZ_CP069062
Organism Mycobacterium tuberculosis strain N0069

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN151_RS14725 Protein ID WP_003414166.1
Coordinates 3107644..3108000 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN151_RS14730 Protein ID WP_003901465.1
Coordinates 3107984..3108214 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN151_RS14705 3103096..3104784 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN151_RS14710 3104788..3105114 - 327 WP_003414157.1 hypothetical protein -
JN151_RS14715 3105287..3105874 + 588 WP_003914429.1 DUF3558 family protein -
JN151_RS14720 3105893..3107542 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN151_RS14725 3107644..3108000 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN151_RS14730 3107984..3108214 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN151_RS14735 3108257..3109300 - 1044 WP_244749321.1 DUF2293 domain-containing protein -
JN151_RS14740 3109299..3109766 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN151_RS14745 3109942..3110196 - 255 WP_003917684.1 hypothetical protein -
JN151_RS14750 3110344..3110748 + 405 WP_003414181.1 hypothetical protein -
JN151_RS14755 3110745..3110936 + 192 WP_003414184.1 hypothetical protein -
JN151_RS14760 3111153..3111413 + 261 Protein_2913 transposase -
JN151_RS14765 3112523..3112690 + 168 Protein_2914 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T188911 WP_003414166.1 NZ_CP069062:c3108000-3107644 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T188911 NZ_CP090592:168691-168966 [Pectobacterium aroidearum]
ATGCAGGGAAAAATAGCCAGTCTACGAAAAAAGCAGCGAGAAACGCTGAGCCAAATTTTCAAAACACCCGTGCTTTCAGG
TGTTAAATGGTCAAATGTTGAGTCACTCATCACTGCGTTAGGTGGTGAAATTAAGGAAGGTAGCGGGTCACGGGTGCGAT
TTTTATTGAATGGGAGTATCGCCCGGTTCCATCGTCCTCACCCGTCACCGGATACTGATAAAGGCGCATTGGTTAACCTG
CGTGAATGGTTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT188911 WP_003901465.1 NZ_CP069062:c3108214-3107984 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT188911 NZ_CP090592:168963-169325 [Pectobacterium aroidearum]
ATGACTAAAGCACTTAACACACCAAATACGATGGTTGTTTCCGGCCAACCGGCCATTATCAGCTATGTACCGGAAATCGG
TATGTTTCGCGGTAAGTTTATTGGCTTATCTGGCTACTGTGATTTTGTCGCTGACAGTATTAATGGGCTGAGAAACGAGG
GCGAAATTTCGCTACGTGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAACGTGAAGAGAAGGTGAAA
ACGTTCACTCTCCGTTATCCTGAATCATTCGGAGAACGACTGACTATTGCGGCGGCTGAACGTCAAATTTCAGTGAATAC
GTTTATTGTCGAAACGCTTAATGAACGTATGAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References