Detailed information of TA system TA188909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3067646..3068325
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN151_RS14495
• Protein ID: WP_003414059.1
• Coordinates: 3067646..3068062 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN151_RS14500
• Protein ID: WP_003414061.1
• Coordinates: 3068059..3068325 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS14470	3063698..3064600	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN151_RS14475	3064669..3065421	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN151_RS14485	3065665..3065940	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN151_RS14490	3065937..3067559	-	1623	WP_003414057.1	class I SAM-dependent DNA methyltransferase	-
JN151_RS14495	3067646..3068062	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN151_RS14500	3068059..3068325	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN151_RS14505	3068351..3068746	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN151_RS14510	3068743..3069012	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN151_RS14515	3069022..3070116	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN151_RS14520	3070113..3070532	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN151_RS14525	3070531..3070605	+	75	Protein_2866	hypothetical protein	-
JN151_RS14530	3070606..3071085	-	480	WP_003414073.1	dihydrofolate reductase	-
JN151_RS14535	3071156..3071956	-	801	WP_244749319.1	thymidylate synthase	-
JN151_RS14540	3072112..3072849	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T188909 WP_003414059.1 NZ_CP069062:c3068062-3067646 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T188909 NZ_CP090591:4749097-4749384 [Pectobacterium aroidearum]
ATGAGCTATAGCGTCAAATTCAGGGAAGATGCGCTGAAAGAATGGCTCAAGCTCGATAAAACCATCCAGCAGCAATTTGC
CAAAAAACTAAAAAAATGCTGCGAAAATCCCCATATTCCTTCGGCAAAATTACGGGGGATGAAGGACTGCTACAAAATAA
AGCTCCGAGCATCAGGCTTTCGTTTGGTTTATGAAGTCATTGACGATGTATTGATTATCGCCGTAGTGGCGGTAGGTAAA
CGTGAACGCAGTGGCGTTTATCATCTGGCGAGTGAGCGAATGAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT188909 WP_003414061.1 NZ_CP069062:c3068325-3068059 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT188909 NZ_CP090591:4748849-4749100 [Pectobacterium aroidearum]
ATGCCGAATATTATATTGAGTGATACCAGTGCCAGCGTCAGTGAATTGAAAAAGAACCCGATGGCGACAGTCAATGCTGG
CGACGGTCTACCTGTCGCTATTTTGAACCGCAACCAGCCTGCGTTTTATTGCATTCCTGCGGATCTCTACGAAAAAATGC
TGGATGCGCTGGACGATCAAGAACTGGTTAAGCTAATCGGTGAACGCAAAAACCAGCCACTGGTTGACGTGGATTTGGAT
AGTTTCTTATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64