Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2878267..2878904 Replicon chromosome
Accession NZ_CP069062
Organism Mycobacterium tuberculosis strain N0069

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN151_RS13475 Protein ID WP_003413180.1
Coordinates 2878267..2878662 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN151_RS13480 Protein ID WP_003413183.1
Coordinates 2878659..2878904 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN151_RS13435 2874352..2875011 + 660 WP_003900846.1 LppA family lipoprotein -
JN151_RS13440 2875008..2875667 + 660 WP_003900847.1 LppA family lipoprotein -
JN151_RS13445 2875664..2876326 + 663 WP_055351404.1 LppA family lipoprotein -
JN151_RS13450 2876323..2876601 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN151_RS13455 2876694..2877107 + 414 WP_003413164.1 PIN domain nuclease -
JN151_RS13460 2877146..2877403 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN151_RS13465 2877400..2877777 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN151_RS13470 2877793..2878167 - 375 WP_003413177.1 hypothetical protein -
JN151_RS13475 2878267..2878662 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN151_RS13480 2878659..2878904 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN151_RS13485 2879315..2879734 - 420 WP_003413190.1 A24 family peptidase -
JN151_RS13490 2879746..2880555 - 810 WP_003413193.1 shikimate dehydrogenase -
JN151_RS13495 2880552..2881808 - 1257 WP_014585306.1 endolytic transglycosylase MltG -
JN151_RS13500 2881801..2882313 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T188907 WP_003413180.1 NZ_CP069062:c2878662-2878267 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T188907 NZ_CP090591:4298166-4298420 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT188907 WP_003413183.1 NZ_CP069062:c2878904-2878659 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT188907 NZ_CP090591:4297918-4298169 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAGAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References