Detailed information of TA system TA188906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2877146..2877777
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: JN151_RS13465
• Protein ID: WP_003413174.1
• Coordinates: 2877400..2877777 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN151_RS13460
• Protein ID: WP_003413167.1
• Coordinates: 2877146..2877403 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS13425	2872404..2872718	+	315	WP_009937839.1	hypothetical protein	-
JN151_RS13430	2873014..2874225	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN151_RS13435	2874352..2875011	+	660	WP_003900846.1	LppA family lipoprotein	-
JN151_RS13440	2875008..2875667	+	660	WP_003900847.1	LppA family lipoprotein	-
JN151_RS13445	2875664..2876326	+	663	WP_055351404.1	LppA family lipoprotein	-
JN151_RS13450	2876323..2876601	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN151_RS13455	2876694..2877107	+	414	WP_003413164.1	PIN domain nuclease	-
JN151_RS13460	2877146..2877403	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN151_RS13465	2877400..2877777	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN151_RS13470	2877793..2878167	-	375	WP_003413177.1	hypothetical protein	-
JN151_RS13475	2878267..2878662	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN151_RS13480	2878659..2878904	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN151_RS13485	2879315..2879734	-	420	WP_003413190.1	A24 family peptidase	-
JN151_RS13490	2879746..2880555	-	810	WP_003413193.1	shikimate dehydrogenase	-
JN151_RS13495	2880552..2881808	-	1257	WP_014585306.1	endolytic transglycosylase MltG	-
JN151_RS13500	2881801..2882313	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T188906 WP_003413174.1 NZ_CP069062:2877400-2877777 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T188906 NZ_CP090591:4297918-4298169 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAGAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT188906 WP_003413167.1 NZ_CP069062:2877146-2877403 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT188906 NZ_CP090591:4298166-4298420 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5