Detailed information of TA system TA188898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2265417..2266043
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN151_RS10590
• Protein ID: WP_003410075.1
• Coordinates: 2265645..2266043 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN151_RS10585
• Protein ID: WP_003911750.1
• Coordinates: 2265417..2265644 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS10575	2263456..2263800	-	345	WP_003410065.1	ferredoxin family protein	-
JN151_RS10580	2263989..2265158	-	1170	WP_244749445.1	ATP-binding protein	-
JN151_RS10585	2265417..2265644	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN151_RS10590	2265645..2266043	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JN151_RS10595	2266226..2266657	-	432	WP_244749299.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN151_RS10600	2266758..2267192	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN151_RS10605	2267629..2267808	-	180	Protein_2094	hypothetical protein	-
JN151_RS10610	2267896..2268516	+	621	WP_003410086.1	IS110 family transposase	-
JN151_RS10615	2268470..2269060	+	591	WP_003899131.1	IS110 family transposase	-
JN151_RS10620	2269188..2270444	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T188898 WP_003410075.1 NZ_CP069062:2265645-2266043 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T188898 NZ_CP090591:c211701-211522 [Pectobacterium aroidearum]
ATGGATAGCAGGACGTTAATCGCAGAAATCAAGGCCGATGGGTGGGAGCTGATACGAGTGAACGGAAGCCACCACCATTT
TATGCACCCCAGCAAACCGGGATTAGTCACAATCCCCCACCCCAAGAAAGATTTACCAATAGGTACGGTAAAAAGCATCA
GGAAACAGGCAGGAATATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT188898 WP_003911750.1 NZ_CP069062:2265417-2265644 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT188898 NZ_CP090591:c211494-211084 [Pectobacterium aroidearum]
ATGTTTTATCCCATTGCAATTGAAGCAGGCGACGATACTCACGCATACGGCGTAACAGTACCAGATTTACCGGGCTGTTT
TTCTGCAGGCGATACGCTAGACGATGCTATCGCGAATGCTAAAGAAGCGATTACCGGACATATCGAGCTACTGGTTGAGA
TGGGTCAGGATATTCCCACCGTATCAACAGTAGGTCAGTTGGCAAAAAGGTCTGAGTATGCAGGGTACACGTGGGCGGTT
GTTGATATTGATGTAACTCGTTTAATGGGCGGCTCAGAAAAAATTAACGTTACATTGCCCAAATCGCTGATTGACCGCAT
TGATCGTTGCGTGGCGAGTAATCCAGAATTTAAAAGTCGTTCGGGTTTTCTTGCACAGGCGGCATTAGAGCGAATTTCGT
CTTCCCGATGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8