Detailed information of TA system TA188892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197629..2198332
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN151_RS10180
• Protein ID: WP_003409778.1
• Coordinates: 2197629..2197958 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN151_RS10185
• Protein ID: WP_003409780.1
• Coordinates: 2197955..2198332 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS10160	2194012..2195082	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN151_RS10165	2195079..2195594	+	516	WP_003409718.1	flavin reductase family protein	-
JN151_RS10170	2195591..2196652	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN151_RS10175	2196649..2197419	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN151_RS10180	2197629..2197958	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN151_RS10185	2197955..2198332	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN151_RS10190	2198329..2198919	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
JN151_RS10195	2198974..2200338	+	1365	WP_014585532.1	HNH endonuclease signature motif containing protein	-
JN151_RS10200	2200493..2200945	-	453	WP_003899095.1	lipoprotein	-
JN151_RS10205	2201009..2201410	+	402	WP_003409869.1	hypothetical protein	-
JN151_RS10210	2201403..2201585	-	183	WP_003409870.1	hypothetical protein	-
JN151_RS10215	2201699..2202049	-	351	WP_003409871.1	hypothetical protein	-
JN151_RS10220	2202060..2202962	-	903	WP_003409874.1	hypothetical protein	-
JN151_RS10225	2202983..2203174	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T188892 WP_003409778.1 NZ_CP069062:c2197958-2197629 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T188892 NZ_CP090590:4274078-4274329 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAGAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT188892 WP_003409780.1 NZ_CP069062:c2198332-2197955 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT188892 NZ_CP090590:4274326-4274580 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8