Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1690794..1691410 Replicon chromosome
Accession NZ_CP069062
Organism Mycobacterium tuberculosis strain N0069

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JN151_RS07945 Protein ID WP_003407593.1
Coordinates 1691093..1691410 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag JN151_RS07940 Protein ID WP_003900349.1
Coordinates 1690794..1691096 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN151_RS07930 1686680..1688527 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JN151_RS07935 1688528..1690780 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JN151_RS07940 1690794..1691096 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JN151_RS07945 1691093..1691410 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JN151_RS07950 1691407..1692411 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JN151_RS07955 1692464..1693753 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
JN151_RS07960 1693826..1694443 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
JN151_RS07965 1694657..1694869 - 213 WP_003898900.1 dodecin family protein -
JN151_RS07970 1694930..1695328 + 399 WP_003900351.1 hypothetical protein -
JN151_RS07975 1695373..1696401 + 1029 WP_003903478.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T188888 WP_003407593.1 NZ_CP069062:1691093-1691410 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T188888 NZ_CP090590:3745307-3745612 [Pectobacterium aroidearum]
ATGCAATTCATTGTTTATGAATATAAACGCGCCAGCCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGACAC
ACCAAAGCGGCGCATGGTCATCCCGCTTATCGAAGCACATCACCTGTCTGAGAAAGTGAATAAGACGTTGTTCCCTCTGA
TCCGCATCGATGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAAGTCATTGGTGAGGCTATC
GCGGATCTTGGCGACTACGCGGATGAGGTCAAAGATGCTATTAATCTGATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT188888 WP_003900349.1 NZ_CP069062:1690794-1691096 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT188888 NZ_CP090590:3745086-3745304 [Pectobacterium aroidearum]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAGTTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAACGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAAGAATGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAACATGGCTCCTTTGCGGATGAAAACAGGAACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References