Detailed information of TA system TA188879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1017263..1017882
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: JN151_RS04810
• Protein ID: WP_003404726.1
• Coordinates: 1017448..1017882 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: JN151_RS04805
• Protein ID: WP_003404724.1
• Coordinates: 1017263..1017442 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS04790	1012736..1014316	+	1581	WP_003909313.1	serine hydrolase	-
JN151_RS04795	1014313..1016706	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
JN151_RS04800	1016979..1017137	+	159	WP_003404720.1	hypothetical protein	-
JN151_RS04805	1017263..1017442	+	180	WP_003404724.1	antitoxin	Antitoxin
JN151_RS04810	1017448..1017882	+	435	WP_003404726.1	SRPBCC family protein	Toxin
JN151_RS04815	1017980..1018753	+	774	WP_244749406.1	VOC family protein	-
JN151_RS04820	1018818..1019267	+	450	WP_003404738.1	hypothetical protein	-
JN151_RS04825	1019402..1019632	-	231	WP_003898642.1	hypothetical protein	-
JN151_RS04830	1019799..1021307	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
JN151_RS04835	1021309..1022547	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T188879 WP_003404726.1 NZ_CP069062:1017448-1017882 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T188879 NZ_CP090589:4298166-4298420 [Pectobacterium aroidearum]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT188879 WP_003404724.1 NZ_CP069062:1017263-1017442 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT188879 NZ_CP090589:4297918-4298169 [Pectobacterium aroidearum]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAGAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAAGCCTGCGTGCTGATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: G0TFQ5