Detailed information of TA system TA188869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 700023..700669
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN151_RS03140
• Protein ID: WP_003403137.1
• Coordinates: 700023..700436 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN151_RS03145
• Protein ID: WP_003403139.1
• Coordinates: 700433..700669 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS03120	696106..697656	+	1551	WP_003403119.1	MlaD family protein	-
JN151_RS03125	697708..698100	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN151_RS03130	698097..698354	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN151_RS03135	698537..699772	-	1236	WP_003403128.1	ATP-binding protein	-
JN151_RS03140	700023..700436	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN151_RS03145	700433..700669	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN151_RS03150	700773..701279	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN151_RS03155	701393..701923	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN151_RS03160	701907..702602	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN151_RS03165	702725..703036	+	312	WP_003403164.1	hypothetical protein	-
JN151_RS03170	703108..704058	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
JN151_RS03175	704299..704883	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
JN151_RS03180	704885..705595	+	711	Protein_630	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T188869 WP_003403137.1 NZ_CP069062:c700436-700023 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T188869 NZ_CP090589:173940-174215 [Pectobacterium aroidearum]
ATGCAGGGAAAAATAGCCAGTCTACGAAAAAAGCAGCGAGAAACGCTGAGCCAAATTTTCAAAACACCCGTGCTTTCAGG
TGTTAAATGGTCAAATGTTGAGTCACTCATCACTGCGTTAGGTGGTGAAATTAAGGAAGGTAGCGGGTCACGGGTGCGAT
TTTTATTGAATGGGAGTATCGCCCGGTTCCATCGTCCTCACCCGTCACCGGATACTGATAAAGGCGCATTGGTTAACCTG
CGTGAATGGTTGGAAAGTATAGGAGTAAAACCATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT188869 WP_003403139.1 NZ_CP069062:c700669-700433 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT188869 NZ_CP090589:174212-174574 [Pectobacterium aroidearum]
ATGACTAAAGCACTTAACACACCAAATACGATGGTTGTTTCCGGCCAACCGGCCATTATCAGCTATGTACCGGAAATCGG
TATGTTTCGCGGTAAGTTTATTGGCTTATCTGGCTACTGTGATTTTGTCGCTGACAGTATTAATGGGCTGAGAAACGAGG
GCGAAATTTCGCTACGTGAATATCTGGAAGACTGTCAGGAAAATGGCATTGAACCTTACGAACGTGAAGAGAAGGTGAAA
ACGTTCACTCTCCGTTATCCTGAATCATTCGGAGAACGACTGACTATTGCGGCGGCTGAACGTCAAATTTCAGTGAATAC
GTTTATTGTCGAAACGCTTAATGAACGTATGAAACAGGCTTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89