Detailed information of TA system TA188866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 643071..643747
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: JN151_RS02890
• Protein ID: WP_003898500.1
• Coordinates: 643071..643484 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: JN151_RS02895
• Protein ID: WP_003402915.1
• Coordinates: 643481..643747 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS02860	638416..638718	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
JN151_RS02865	638778..639056	-	279	WP_003402901.1	hypothetical protein	-
JN151_RS02870	639053..640306	-	1254	WP_003402904.1	inorganic phosphate transporter	-
JN151_RS02875	640426..640812	-	387	WP_003402907.1	VOC family protein	-
JN151_RS02880	640875..641759	-	885	WP_003402909.1	SDR family oxidoreductase	-
JN151_RS02885	641855..642757	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
JN151_RS02890	643071..643484	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN151_RS02895	643481..643747	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JN151_RS02900	643939..645654	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
JN151_RS02905	645732..647336	+	1605	WP_244749391.1	amidohydrolase	-
JN151_RS02910	647333..648313	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T188866 WP_003898500.1 NZ_CP069062:c643484-643071 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T188866 NZ_CP090588:c4581855-4581568 [Pectobacterium aroidearum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGTTAGGGCATCCTGTCAGGGAACAATTCAA
AAAAAAGCTAGTTGAGCGTTTAGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGTCGTAAAGACCAGTATAAAATTA
AGCTGAAGTCTGCTGGTTACAGGCTGGTATATTTGGTTCAGGATGAGACCATTACGGTGATGATCATGGGGGTTGGAAAG
CGCGAAGGTAGTCAGGTTTATTTTGATACTAAAGGCCGCTCTGCTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT188866 WP_003402915.1 NZ_CP069062:c643747-643481 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT188866 NZ_CP090588:c4582099-4581845 [Pectobacterium aroidearum]
ATGGCTTTCAATATCCTAACTAACACAGCGGCCAGCATCACTGAACTGAAACGCGACCCAATGGGGACAGTACGGGCTGC
TGAAGGTGAGACTATCGCTATACTGAACCGGAATGAGCCTGCGTTCTATTGTGTGCCTCCTGCCATGTTCGCTTATTTAA
TGGAATTGGCTGAAGATGCAGAATTGGGGCGTACTGTTGATGATCGTATGTCAGAGTTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0