Detailed information of TA system TA188861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 71284..71917
• Replicon: chromosome
• Accession: NZ_CP069062
• Organism: Mycobacterium tuberculosis strain N0069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFC0
• Locus tag: JN151_RS00360
• Protein ID: WP_003400580.1
• Coordinates: 71516..71917 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN151_RS00355
• Protein ID: WP_244749369.1
• Coordinates: 71284..71523 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN151_RS00340	66581..68020	+	1440	WP_003902831.1	FAD-binding oxidoreductase	-
JN151_RS00345	68076..68237	+	162	WP_003899796.1	hypothetical protein	-
JN151_RS00350	68278..71217	+	2940	WP_003901785.1	UPF0182 family protein	-
JN151_RS00355	71284..71523	+	240	WP_244749369.1	antitoxin	Antitoxin
JN151_RS00360	71516..71917	+	402	WP_003400580.1	type II toxin-antitoxin system ribonuclease VapC1	Toxin
JN151_RS00365	71969..74206	-	2238	WP_244749370.1	NADP-dependent isocitrate dehydrogenase	-
JN151_RS00370	74324..74893	-	570	WP_003902834.1	TetR/AcrR family transcriptional regulator	-
JN151_RS00375	74996..75907	+	912	WP_003899798.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (36-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14339.44 Da
• Isoelectric Point: 4.8887

>T188861 WP_003400580.1 NZ_CP069062:71516-71917 [Mycobacterium tuberculosis]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Nucleotide

• Length: 402 bp

>T188861 NZ_CP090588:c4118686-4118273 [Pectobacterium aroidearum]
GTGGCCCAGTGGCAATGTGATCTTCGCGTTTCCTGGCGTATGCAACTGCTTTCATTGATTGTGCATGGCCTATTAGTATT
GCTCATTCTTCTGGCGCCGTGGCCGGACGGCTATGCATGGCTGTGGCTATGCCTGGTCACGATGGTGATGTTCGGTTTTA
TCCGTAGCCAAAGGAATATCAAATCCCGGCAGGGGGAAATCGTTTTGCTCAGCGAAACGACCCTGAACTGGCGGCAGCAG
GAATGGCAGATCGTTAAACGACCGTGGTTGCTGAAAAATGGCGTATTGCTGTCATTGCAGGCAGTGAACGGTAAAGACAG
ACAACAGCTGTGGCTGGCATCGGACAGCATGGGCGATGACGAATGGCGTCATCTGCGTCAGCTTCTTTTGCAGCAGAAAA
ACTGGGCGAGATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8744.87 Da
• Isoelectric Point: 8.5960

>AT188861 WP_244749369.1 NZ_CP069062:71284-71523 [Mycobacterium tuberculosis]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALANTASPGISRETIEASRRELRGG

Nucleotide

• Length: 240 bp

>AT188861 NZ_CP090588:c4118933-4118667 [Pectobacterium aroidearum]
ATGGAAATCGATAATAAATCACGCATTCATTGGGCATGCCGCCGGGGTATGCGTGAGTTGGATATCGCGATCATGCCGTT
TTTTGAGCATGACTACGACACACTCAGTGACAGCGATAAGCGTACCTTTGTGCGCCTGTTACAAAGCGATGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCGAGCCGAAAGACGGAGAGCTGAAACGCATGATTTCTCTTATTCAAACGCGAAAT
AAAGATCGTGGCCCAGTGGCAATGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BR82