Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41269..41912 Replicon plasmid p33Kpn22-5
Accession NZ_CP069051
Organism Klebsiella pneumoniae strain 33Kpn22

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag IFY68_RS30400 Protein ID WP_000754566.1
Coordinates 41269..41685 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag IFY68_RS30405 Protein ID WP_001261276.1
Coordinates 41682..41912 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IFY68_RS30385 36527..39532 + 3006 WP_001143775.1 Tn3-like element Tn3 family transposase -
IFY68_RS30390 39565..39927 - 363 Protein_42 transposase -
IFY68_RS30395 40209..41065 - 857 Protein_43 IS3-like element ISEc15 family transposase -
IFY68_RS30400 41269..41685 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
IFY68_RS30405 41682..41912 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IFY68_RS30410 42486..42836 + 351 WP_000493378.1 hypothetical protein -
IFY68_RS30415 42887..43630 + 744 WP_000129823.1 hypothetical protein -
IFY68_RS30420 43627..44100 + 474 WP_087868098.1 tyrosine-type recombinase/integrase -
IFY68_RS30425 44189..44377 + 189 WP_000957857.1 hypothetical protein -
IFY68_RS30430 44387..45586 + 1200 WP_000948429.1 IS91 family transposase -
IFY68_RS30435 45759..46091 + 333 Protein_51 tyrosine-type recombinase/integrase -
IFY68_RS30440 46149..46406 - 258 WP_000764642.1 hypothetical protein -
IFY68_RS30445 46535..46639 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aac(6')-Ib - 1..47426 47426
- inside IScluster/Tn aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aac(6')-Ib - 7476..45586 38110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T188858 WP_000754566.1 NZ_CP069051:c41685-41269 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T188858 NZ_CP090588:c3200330-3200016 [Pectobacterium aroidearum]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGTTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAATTGAATCGTCGGATAGTGATCCCGTTGCTCCCGATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCGGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGGCCCTGGGG
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCTCAGGTAAAGGCTGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT188858 WP_001261276.1 NZ_CP069051:c41912-41682 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT188858 NZ_CP090588:c3200569-3200333 [Pectobacterium aroidearum]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
TATTAACCTGAGCGCTACCCTGACAACCGCCCTAGATGCAGAGCTTCGCTATCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAAGGAAGCTCTCGCAGCACTAAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References